Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,667,617	(A)6→5	intergenic (‑37/+174)	gadA ← / ← ccp	glutamate decarboxylase A/cytochrome c peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,667,612	0	A	.	100.0%	69.3 / NA	17	intergenic (‑32/+179)	gadA/ccp	glutamate decarboxylase A/cytochrome c peroxidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (7/10);  total (7/10)

AGCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAATAAAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACAAAATCCTAAGCAG  >  NC_000913/3667550‑3667683                                                               |                                                                        aGCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTAt                                                         <  1:702556/78‑1 (MQ=255)  gCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCg                                                      >  2:702581/1‑80 (MQ=255)    ggAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCaa                                                   >  1:702584/1‑81 (MQ=255)          ccGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAg                                             >  1:702579/1‑81 (MQ=255)             ttAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCaa                                          <  2:702557/81‑1 (MQ=255)                aCAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGc                                       <  2:702558/81‑1 (MQ=255)                       cTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTAc                                  <  1:702560/79‑1 (MQ=255)                       cTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACa                                 <  2:702559/80‑1 (MQ=255)                       cTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACa                                 <  2:702561/80‑1 (MQ=255)                                  cGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTa                      >  2:702562/1‑80 (MQ=255)                                    aaCTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTaaa                    >  1:702587/1‑80 (MQ=255)                                            aaaTTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACaaaa            <  1:702563/80‑1 (MQ=255)                                            aaaTTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACAAAAt           >  2:702586/1‑81 (MQ=255)                                                   tttGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACAAAATCCTAAGc    <  1:702565/81‑1 (MQ=255)                                                   tttGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACAAAATCCTAAGc    <  2:702564/81‑1 (MQ=255)                                                    ttGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACAAAATCCTAAg     >  2:702588/1‑79 (MQ=255)                                                      gAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACAAAATCCTAAGCAg  <  2:702566/80‑1 (MQ=255)                                                               |                                                                        AGCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAATAAAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACAAAATCCTAAGCAG  >  NC_000913/3667550‑3667683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑