Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,747,446	(A)5→4	coding (363/1497 nt)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,747,442	0	A	.	100.0%	140.3 / NA	33	coding (359/1497 nt)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (16/17);  total (16/17)

CCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGC  >  NC_000913/3747363‑3747516                                                                                |                                                                           ccGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGaa                                                                           <  1:722088/81‑1 (MQ=255)    cTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGaaaac                                                                        <  1:722089/81‑2 (MQ=255)    cTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGaaaac                                                                        <  1:722090/81‑2 (MQ=255)      cGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGaaaactc                                                                      >  2:722156/1‑78 (MQ=255)      cGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGaaaactc                                                                      >  1:722154/1‑78 (MQ=255)      cGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGaaaactc                                                                      >  1:722143/1‑78 (MQ=255)       gggAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGaaaact                                                                       <  1:722091/79‑3 (MQ=255)         gAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTAc                                                                  >  2:722155/1‑81 (MQ=255)            tGCTATTTTGTCCTCGGACCGCCAGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCg                                                               <  2:722092/81‑1 (MQ=255)             gCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGc                                                              >  2:722145/1‑81 (MQ=255)                 ttttGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTg                                                            <  1:722093/79‑1 (MQ=255)                 ttttGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGAc                                                          <  2:722094/81‑1 (MQ=255)                     gTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGa                                                      >  2:722151/1‑81 (MQ=255)                     gTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGa                                                      >  2:722152/1‑81 (MQ=255)                           ggACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAAcc                                                >  2:722144/1‑81 (MQ=255)                            gACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCt                                               >  1:722140/1‑81 (MQ=255)                               cgccgGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTtgt                                            >  2:722149/1‑81 (MQ=255)                                gccgGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTtgtg                                           >  2:722171/1‑81 (MQ=255)                                     gCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCg                                      <  2:722095/81‑1 (MQ=255)                                      cGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCgg                                     <  1:722096/81‑1 (MQ=255)                                           gAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCAt                                 <  1:722097/80‑1 (MQ=255)                                            aaaTCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATcc                               <  2:722098/81‑1 (MQ=255)                                            aaaTCGTTCGTCGCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATcc                               <  1:722099/81‑1 (MQ=255)                                                   tcgtcgCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCg                        >  2:722167/1‑81 (MQ=255)                                                     gtcgCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCt                      >  1:722162/1‑81 (MQ=255)                                                     gtcgCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCt                      >  1:722153/1‑81 (MQ=255)                                                        gCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGtt                   <  1:722100/81‑1 (MQ=255)                                                        gCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGtt                   <  2:722101/81‑1 (MQ=255)                                                        gCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGt                    >  1:722150/1‑80 (MQ=255)                                                        gCTGGCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGt                    >  1:722157/1‑80 (MQ=255)                                                            gCAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACg                <  1:722102/80‑1 (MQ=255)                                                             cAGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgct              <  2:722103/81‑1 (MQ=255)                                                              aGGAAGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgctg             >  2:722146/1‑81 (MQ=255)                                                                  aGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgctggctg         <  2:722105/81‑1 (MQ=255)                                                                  aGATGGCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgctggctg         <  2:722104/81‑1 (MQ=255)                                                                      ggCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGa        >  2:722147/1‑78 (MQ=255)                                                                       gCATCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAaga    <  2:722106/81‑1 (MQ=255)                                                                       gCAACCCGG‑AAAACTCTACCCGGTGGACCGAAAAAACTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAag     <  1:722108/80‑1 (MQ=255)                                                                         aTCCCGG‑AAAACTCTACCCGCTGGCCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGc  <  2:722107/81‑1 (MQ=255)                                                                         aTCCCGG‑AAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAagag   >  1:722170/1‑80 (MQ=255)                                                                                |                                                                           CCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGC  >  NC_000913/3747363‑3747516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑