Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,797,041	(T)8→7	coding (94/1260 nt)	waaL →	O‑antigen ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,797,034	0	T	.	100.0%	56.2 / NA	14	coding (87/1260 nt)	waaL	O‑antigen ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (4/10);  total (4/10)

CTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTT  >  NC_000913/3796959‑3797106                                                                            |                                                                         cTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGtttttt                                                                     <  2:734598/81‑1 (MQ=255)   ttAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGttttttt                                                                    >  1:734621/1‑80 (MQ=255)       aCTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGtttttttcaat                                                                >  2:734620/1‑76 (MQ=255)                 ttGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATG‑TTTTTTTCAATGATAATTGCAt                                                    <  2:734599/81‑1 (MQ=255)                      aCCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATG‑TTTTTTTCAATGATAATTGCATtcgtc                                               <  2:734600/81‑1 (MQ=255)                           acacTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATG‑TTTTTTTCAATGATAATTGCATTCGTCGata                                           <  1:734601/80‑1 (MQ=255)                            cacTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATG‑TTTTTTTCAATGATAATTGCATTCGTCGataa                                          <  2:734602/80‑1 (MQ=255)                             acTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATG‑TTTTTTTCAATGATAATTGCATTCGTCGataata                                        >  1:734628/1‑81 (MQ=255)                                   aaaTCATCAATGATTTTAGAGATAATAACTTATATATTATG‑TTTTTTTCAATGATAATTGCATTCGTCGATAATACtt                                     <  1:734603/78‑1 (MQ=255)                                        atcaATGATTTTAGAGATAATAACTTATATATTATG‑TTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCAt                              <  2:734604/80‑1 (MQ=255)                                               aTTTTAGAGATAATAACTTATATATTATG‑TTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAatat                      <  1:734605/81‑1 (MQ=255)                                                    agagATAATAACTTATATATTATG‑TTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATataa                  >  2:734625/1‑80 (MQ=255)                                                         taataaCTTATATATTATG‑TTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCAc            >  1:734606/1‑81 (MQ=255)                                                           ataaCTTATATATTATG‑TTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACt           <  1:734607/80‑1 (MQ=255)                                                              aCTTATATATTATG‑TTTTTTTCAATGATATTTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCt        <  2:734608/80‑1 (MQ=255)                                                              aCTTATATATTATG‑TTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCt        <  2:734609/80‑1 (MQ=255)                                                                   tatatTATG‑TTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGtt  >  2:734630/1‑81 (MQ=255)                                                                            |                                                                         CTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTT  >  NC_000913/3796959‑3797106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑