Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,797,159	(A)6→5	coding (212/1260 nt)	waaL →	O‑antigen ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,797,154	0	A	.	100.0%	29.0 / NA	8	coding (207/1260 nt)	waaL	O‑antigen ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (2/6);  total (2/6)

AAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTA  >  NC_000913/3797080‑3797229                                                                           |                                                                            aaaaTATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATaaaaac                                                                        <  2:734614/80‑2 (MQ=255)         taataTCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTAttctt                                                              <  2:734615/81‑1 (MQ=255)            tatCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTccc                                                           <  1:734616/81‑1 (MQ=255)                                 aTTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAg                                        <  1:734617/79‑1 (MQ=255)                                 aTTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGgc                                      >  2:734629/1‑81 (MQ=255)                                           aTTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGAtt                            <  1:734618/81‑1 (MQ=255)                                                 cGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAAttt                      <  2:734619/81‑1 (MQ=255)                                                    ggCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTgg                    >  1:734637/1‑80 (MQ=255)                                                      cAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTAt                 <  1:734620/81‑1 (MQ=255)                                                                     taatat‑aaAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTa  <  2:734621/81‑1 (MQ=255)                                                                           |                                                                            AAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTA  >  NC_000913/3797080‑3797229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑