Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,847,479	Δ1 bp	coding (1146/1320 nt)	uhpC ←	inner membrane protein sensing glucose‑6‑phosphate

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,847,476	0	C	.	100.0%	196.2 / NA	45	coding (1149/1320 nt)	uhpC	inner membrane protein sensing glucose‑6‑phosphate
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (25/20);  total (25/20)

TCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAA  >  NC_000913/3847397‑3847549                                                                                |                                                                          tCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAAcc                                                                          >  1:746203/1‑81 (MQ=255)    aGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAAcccgt                                                                       >  1:746220/1‑79 (MQ=255)     gTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAAcccgtc                                                                      <  1:746159/81‑4 (MQ=255)       gCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTcgc                                                                   >  1:746215/1‑81 (MQ=255)         cAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTcgccg                                                                 <  1:746160/81‑1 (MQ=255)              aTCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCt                                                            >  1:746212/1‑81 (MQ=255)               tCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCt                                                            <  2:746161/80‑1 (MQ=255)                cGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTgc                                                          >  1:746225/1‑81 (MQ=255)                cGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTgc                                                          >  1:746207/1‑81 (MQ=255)                 gAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTgcc                                                         <  1:746162/81‑1 (MQ=255)                   gTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTgccgc                                                       >  1:746223/1‑81 (MQ=255)                    tACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTgccgcc                                                      >  1:746231/1‑81 (MQ=255)                     aCTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCct                                                     >  2:746248/1‑81 (MQ=255)                      cTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCctc                                                    <  2:746163/81‑1 (MQ=255)                      cTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCctc                                                    >  1:746232/1‑81 (MQ=255)                         tCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCctct                                                   <  2:746164/79‑1 (MQ=255)                         tCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCtt                                                  <  1:746165/80‑1 (MQ=255)                                cGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGaa                                          <  1:746166/81‑1 (MQ=255)                                 ggCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGaa                                          >  1:746226/1‑80 (MQ=255)                                    cAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACAct                                      >  1:746234/1‑81 (MQ=255)                                     aaCCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACActc                                     <  1:746167/81‑1 (MQ=255)                                        cAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTgc                                  <  2:746168/81‑1 (MQ=255)                                           cAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTgccgc                               >  2:746230/1‑81 (MQ=255)                                           cAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTgccgc                               <  2:746169/81‑1 (MQ=255)                                             aGCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTgccgccg                             >  2:746233/1‑81 (MQ=255)                                              gCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTgccgccgc                            <  1:746170/81‑1 (MQ=255)                                              gCGACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTgccgccgc                            >  1:746224/1‑81 (MQ=255)                                                gACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCa                          >  2:746218/1‑81 (MQ=255)                                                gACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCa                          >  1:746243/1‑81 (MQ=255)                                                gACGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCa                          >  2:746229/1‑81 (MQ=255)                                                 aCGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCa                          >  2:746213/1‑80 (MQ=255)                                                 aCGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCAt                         <  1:746172/81‑1 (MQ=255)                                                 aCGCCCCCAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCAt                         <  1:746171/81‑1 (MQ=255)                                                    cccccAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATAcc                      >  2:746250/1‑81 (MQ=255)                                                     ccccAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATAcc                      >  2:746221/1‑80 (MQ=255)                                                        cAGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGAtt                  <  1:746173/81‑1 (MQ=255)                                                         aGATAAGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGAtt                  >  1:746237/1‑80 (MQ=255)                                                             aaGCAAACAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACAt             >  1:746222/1‑81 (MQ=255)                                                                 aaaCAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCtgt         <  1:746175/81‑1 (MQ=255)                                                                 aaaCAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCtgt         <  1:746174/81‑1 (MQ=255)                                                                  aaCAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCtgtg        <  2:746176/81‑1 (MQ=255)                                                                   aCAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTgg       <  2:746177/81‑1 (MQ=255)                                                                    cAAGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTggg      >  1:746227/1‑81 (MQ=255)                                                                     aaGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGc     >  1:746252/1‑81 (MQ=255)                                                                      aGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGcc    <  1:746178/81‑1 (MQ=255)                                                                      aGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGc     >  2:746211/1‑80 (MQ=255)                                                                      aGCCGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGc     >  2:746238/1‑80 (MQ=255)                                                                        ccGACAAA‑CCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCaa  <  1:746179/81‑1 (MQ=255)                                                                                |                                                                          TCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAA  >  NC_000913/3847397‑3847549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑