Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,869,395	Δ1 bp	coding (19/1065 nt)	cbrA →	colicin M resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,869,394	0	G	.	100.0%	151.0 / NA	35	coding (18/1065 nt)	cbrA	colicin M resistance protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (22/13);  total (22/13)

ACAAGGACTTCACTTTCAGACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGC  >  NC_000913/3869318‑3869465                                                                             |                                                                        acaAGGACTTCACTTTCAGACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTgcga                                                                      <  1:752001/80‑4 (MQ=255)  caAGGACTTCACTTTCAGACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGatt                                                                   <  1:752002/81‑1 (MQ=255)   aaGGACTTCACTTTCAGACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGatta                                                                  <  1:752003/81‑1 (MQ=255)   aaGGACTTCACTTTCAGACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGatta                                                                  >  1:752067/1‑81 (MQ=255)     ggACTTCACTTTCAGACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGattatt                                                                >  2:752043/1‑81 (MQ=255)      gACTTCACTTTCAGACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTg                                                               >  1:752059/1‑81 (MQ=255)         ttCACTTTCAGACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGcc                                                            >  1:752042/1‑81 (MQ=255)          tCACTTTCAGACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCt                                                           >  2:752066/1‑81 (MQ=255)            aCTTTCAGACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCg                                                         >  1:752036/1‑81 (MQ=255)               ttCAGACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGcc                                                      >  2:752051/1‑81 (MQ=255)                 cAGACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCg                                                    >  2:752045/1‑81 (MQ=255)                   gACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCgg                                                   >  1:752053/1‑80 (MQ=255)                        tAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGAt                                             <  2:752004/81‑1 (MQ=255)                         aGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATc                                            >  1:752049/1‑81 (MQ=255)                              tttGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGt                                       >  1:752075/1‑81 (MQ=255)                                  ccATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTgg                                    >  2:752054/1‑80 (MQ=255)                                    aTCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCAc                                 <  1:752006/81‑1 (MQ=255)                                    aTCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCAc                                 <  2:752005/81‑1 (MQ=255)                                     tCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACg                                <  1:752007/81‑1 (MQ=255)                                      cGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACg                                >  1:752046/1‑80 (MQ=255)                                       gTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGa                               >  1:752048/1‑80 (MQ=255)                                                gtgagtgaAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAg                     >  2:752055/1‑81 (MQ=255)                                                 tgagtgaAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAgg                    >  1:752056/1‑81 (MQ=255)                                                   agtgaAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGc                   >  2:752047/1‑80 (MQ=255)                                                    gtgaAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCaa                 <  1:752009/81‑1 (MQ=255)                                                    gtgaAAAGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCaa                 <  1:752008/81‑1 (MQ=255)                                                       aaaaGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAAt              >  1:752074/1‑81 (MQ=255)                                                         aaGATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGc            <  1:752010/81‑1 (MQ=255)                                                           gATGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCATGAAAGTTAGCAGGCAAAATGCAg          >  2:752087/1‑81 (MQ=255)                                                            aTGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAgg         >  1:752073/1‑81 (MQ=255)                                                             tGGAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGt        <  2:752011/81‑1 (MQ=255)                                                               gAACATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGa      >  2:752064/1‑81 (MQ=255)                                                                 aCATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATc    <  2:752012/81‑1 (MQ=255)                                                                  cATTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATcg   <  2:752013/81‑1 (MQ=255)                                                                   aTTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATcgc  <  2:752014/81‑1 (MQ=255)                                                                   aTTTCGACGT‑GCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATcgc  >  2:752070/1‑81 (MQ=255)                                                                             |                                                                        ACAAGGACTTCACTTTCAGACAATAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGC  >  NC_000913/3869318‑3869465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑