Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,853	(T)6→5	intergenic (+24/‑91)	thrT → / → tufB	tRNA‑Thr/translation elongation factor Tu 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,848	0	T	.	100.0%	114.7 / NA	27	intergenic (+19/‑96)	thrT/tufB	tRNA‑Thr/translation elongation factor Tu 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (17/10);  total (17/10)

GTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATC  >  NC_000913/4175768‑4175922                                                                                 |                                                                           gTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGt                                                                            <  1:830133/81‑1 (MQ=255)  ttGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtt                                                                           <  1:830134/81‑1 (MQ=255)   tGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGttt                                                                          >  2:830217/1‑81 (MQ=255)   tGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGttt                                                                          >  2:830204/1‑81 (MQ=255)     gTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGttttt                                                                        <  2:830137/81‑1 (MQ=255)     gTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttt                                                                         <  1:830135/80‑1 (MQ=255)     gTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttt                                                                         <  1:830136/80‑1 (MQ=255)     gTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttt                                                                         <  2:830138/80‑1 (MQ=255)        gagCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttttcct                                                                     <  1:830139/81‑4 (MQ=255)         agCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttttcctt                                                                    <  1:830140/81‑5 (MQ=255)          gcgcACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTc                                                                  >  1:830209/1‑81 (MQ=255)           cgcACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCt                                                                 <  2:830141/81‑1 (MQ=255)             cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGt                                                               >  2:830198/1‑81 (MQ=255)              aCCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGtt                                                              >  2:830203/1‑81 (MQ=255)              aCCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGtt                                                              >  1:830182/1‑81 (MQ=255)              aCCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGtt                                                              <  2:830142/81‑1 (MQ=255)                  ttGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCCTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTAtt                                                          >  2:830202/1‑81 (MQ=255)                    ggTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTg                                                         >  2:830192/1‑80 (MQ=255)                    ggTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTg                                                         <  2:830143/80‑1 (MQ=255)                      tAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCAt                                                      >  1:830179/1‑81 (MQ=255)                              aGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTcaacaa                                               <  1:830144/80‑1 (MQ=255)                                gTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTc                                            >  2:830184/1‑81 (MQ=255)                                    gCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCggg                                         >  1:830191/1‑80 (MQ=255)                                          cGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGtt                                   <  2:830145/80‑1 (MQ=255)                                           gAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGc                                 >  1:830208/1‑81 (MQ=255)                                            aaTCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTTcc                                >  2:830196/1‑81 (MQ=255)                                            aaTCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGcc                                >  1:830210/1‑81 (MQ=255)                                               cTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTgg                             >  1:830219/1‑81 (MQ=255)                                                 gCCTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGtt                           >  2:830189/1‑81 (MQ=255)                                                  ccTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCTGGCATGTTGCCTGGTTg                          <  2:830147/81‑1 (MQ=255)                                                  ccTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTg                          <  2:830146/81‑1 (MQ=255)                                                   cTATCAGCACCACTTCTTTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGa                         >  1:830207/1‑81 (MQ=255)                                                                  cttTTCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCAc           <  2:830148/80‑1 (MQ=255)                                                                     ttCTCCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCg         <  2:830149/79‑1 (MQ=255)                                                                      tctcCTCCCTG‑TTTTTCCTTCTGTTTATTGCATTCTACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGAtt      >  1:830206/1‑81 (MQ=255)                                                                          ctccCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGAttt     <  1:830150/78‑1 (MQ=255)                                                                          ctccCTG‑TTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATc  >  1:830195/1‑81 (MQ=255)                                                                                 |                                                                           GTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATC  >  NC_000913/4175768‑4175922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑