Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,310,656	(T)7→6	coding (325/1533 nt)	alsA ←	D‑allose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,310,650	0	T	.	100.0%	119.4 / NA	28	coding (331/1533 nt)	alsA	D‑allose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (13/15);  total (13/15)

AACTTTCAAGCCCACGCGTAATAACATCATGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGATTTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATCAATAACGCTGAGTTCCTGATA  >  NC_000913/4310570‑4310725                                                                                 |                                                                            aaCTTTCAAGCCCACGCGTAATAACATCATGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGAt                                                                             >  2:864156/1‑81 (MQ=255) aaCTTTCAAGCCCACGCGTAATAACATCATGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGAt                                                                             <  1:864124/81‑1 (MQ=255)   cTTTCAAGCCCACGCGTAATAACATCATGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGAttt                                                                           >  2:864155/1‑81 (MQ=255)       cAAGCCCACGCGTAATAACATCATGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGAtttttt                                                                        <  2:864125/80‑1 (MQ=255)        aaGCCCACGCGTAATAACATCATGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGAttttttc                                                                       <  2:864126/80‑2 (MQ=255)         aGCCCACGCGTAATAACATCATGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGAttttttcgt                                                                     >  2:864157/1‑78 (MQ=255)            ccACGCGTAATAACATCATGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAg                                                                 <  2:864127/81‑1 (MQ=255)              aCGCGTAATAACATCATGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGAt                                                               >  1:864150/1‑81 (MQ=255)                     ataaCATCATGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGAcc                                                        <  1:864128/81‑1 (MQ=255)                       aaCATCATGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTTACGCCACAGA‑TTTTTTCGTCATATGACTACCaa                                                      >  2:864166/1‑81 (MQ=255)                       aaCATCATGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCaa                                                      >  2:864162/1‑81 (MQ=255)                            catGGCGGCGCGGACACGCATTTCTCGCCAGCCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAatata                                                  >  2:864169/1‑80 (MQ=255)                             atGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAatatat                                                 >  2:864159/1‑80 (MQ=255)                                       ggACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAg                                      >  2:864164/1‑81 (MQ=255)                                       ggACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAg                                      >  2:864168/1‑81 (MQ=255)                                        gACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGt                                     <  1:864129/81‑1 (MQ=255)                                          cacGCATTTCTCGCCAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTAc                                   >  1:864176/1‑81 (MQ=255)                                           acGCATTTCTCGCCAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACg                                  <  2:864130/81‑1 (MQ=255)                                            cGCATTTCTCGCCAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACgg                                 <  2:864131/81‑1 (MQ=255)                                              cATTTCTCGCCAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGtt                               <  2:864132/81‑1 (MQ=255)                                                      gCCAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATtc                          <  1:864133/78‑1 (MQ=255)                                                        cAGTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATCaa                     >  2:864175/1‑81 (MQ=255)                                                          gTCGATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATCAATa                   <  1:864134/81‑1 (MQ=255)                                                             gATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATCAATAAc                 <  1:864135/80‑1 (MQ=255)                                                             gATAATATTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATCAATAACg                >  2:864172/1‑81 (MQ=255)                                                              ataataTTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATCAATAACGc               <  1:864136/81‑1 (MQ=255)                                                               taataTTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATCAATAACGCt              <  1:864137/81‑1 (MQ=255)                                                                 atatTGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATCAATAACGCTg             <  1:864138/80‑1 (MQ=255)                                                                    ttGACGCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATAAATAACGCTGAGt          <  2:864139/80‑1 (MQ=255)                                                                         gCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATCAATAACGCTGAGTTCCTGa    >  1:864170/1‑81 (MQ=255)                                                                         gCCACAGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATCAATAACGCTGAGTTCCTGa    >  2:864167/1‑81 (MQ=255)                                                                          ccACAGA‑TTTTTTCGTCAGATGACGACCTATATATAAATTTTCCAGTACGGTTAATTCATCAATAACGCTGAGTTCCTGat   <  2:864140/81‑1 (MQ=255)                                                                           cacaGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATCAATAACGCTGAGTTCCTGata  <  2:864141/81‑1 (MQ=255)                                                                            acaGA‑TTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATCAATAACGCTGAGTTCCTGata  >  2:864165/1‑80 (MQ=255)                                                                                 |                                                                            AACTTTCAAGCCCACGCGTAATAACATCATGGCGGCGCGGACACGCATTTCTCGCCAGTCGATAATATTGACGCCACAGATTTTTTTCGTCAGATGACGACCAATATATAAATTTTCCAGTACGGTTAATTCATCAATAACGCTGAGTTCCTGATA  >  NC_000913/4310570‑4310725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑