Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,523,074	Δ1 bp	coding (1021/1968 nt)	yjhG ←	KpLE2 phage‑like element; D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,523,071	0	G	.	100.0%	168.0 / NA	39	coding (1024/1968 nt)	yjhG	KpLE2 phage‑like element; D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (22/17);  total (22/17)

CTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGG  >  NC_000913/4522993‑4523146                                                                               |                                                                            cTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCggg                                                                           <  2:914779/81‑1 (MQ=255)   tCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCgggca                                                                         >  2:914844/1‑79 (MQ=255)   tCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCgggca                                                                         >  2:914827/1‑79 (MQ=255)     cGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCgggcac                                                                        <  1:914781/80‑4 (MQ=255)     cGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCgggcac                                                                        <  2:914780/80‑4 (MQ=255)      ggCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCAcgc                                                                     <  1:914782/81‑1 (MQ=255)      ggCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCAcgc                                                                     <  1:914783/81‑1 (MQ=255)         acacCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCt                                                                  <  1:914784/81‑1 (MQ=255)              accTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCttgtt                                                              >  2:914864/1‑80 (MQ=255)                cTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGAt                                                           >  2:914826/1‑81 (MQ=255)                  tccATAAAGGCATTGACCGTTGGCTTCTCCCCCGGGCCATTCGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGc                                                         >  1:914840/2‑81 (MQ=255)                       aaaGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATc                                                    >  2:914852/1‑81 (MQ=255)                       aaaGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATc                                                    >  1:914884/1‑81 (MQ=255)                        aaGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATc                                                    <  2:914785/80‑1 (MQ=255)                        aaGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATc                                                    >  1:914832/1‑80 (MQ=255)                           gCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGt                                                >  1:914845/1‑81 (MQ=255)                            cATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGtc                                               <  1:914786/81‑1 (MQ=255)                             aTTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGtca                                              <  2:914787/81‑1 (MQ=255)                              ttGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGtcat                                             <  1:914788/81‑1 (MQ=255)                              ttGACCGTTGGATGATAAACCAGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGtcat                                             <  2:914789/81‑1 (MQ=255)                                gACCGTTGGATGATAAGCCGGGCCGTTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGtcatc                                            <  1:914790/80‑1 (MQ=255)                                gACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGtcatc                                            >  2:914854/1‑80 (MQ=255)                                 aCCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCaa                                          >  2:914851/1‑81 (MQ=255)                                 aCCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCaa                                          <  1:914791/81‑1 (MQ=255)                                    gTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACgg                                       >  2:914847/1‑81 (MQ=255)                                    gTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACgg                                       >  2:914843/1‑81 (MQ=255)                                     ttGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGt                                      >  2:914849/1‑81 (MQ=255)                                       ggATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTc                                     <  2:914792/80‑1 (MQ=255)                                       ggATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCg                                    >  2:914846/1‑81 (MQ=255)                                           gatAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGa                                 >  2:914856/1‑80 (MQ=255)                                           gatAAACCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGa                                 >  2:914865/1‑80 (MQ=255)                                               aaCCGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATgg                            >  2:914842/1‑81 (MQ=255)                                                 ccGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGc                           >  1:914835/1‑80 (MQ=255)                                                  cGGGCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCaa                         >  1:914867/1‑81 (MQ=255)                                                   gggCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAAc                        >  1:914861/1‑81 (MQ=255)                                                    ggCCATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAAcc                       <  2:914793/81‑1 (MQ=255)                                                      ccATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAAcctg                     >  1:914901/1‑81 (MQ=255)                                                       cATTAGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAAcctgc                    <  2:914794/81‑1 (MQ=255)                                                           aGGCAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAAcctgcctg                 <  1:914795/80‑1 (MQ=255)                                                              cAGTACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGa             <  2:914796/81‑1 (MQ=255)                                                                 tACGCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCa          <  1:914797/81‑1 (MQ=255)                                                                    gCTCACCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAAtt       >  1:914874/1‑81 (MQ=255)                                                                        aCCAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGcc    <  2:914798/80‑1 (MQ=255)                                                                         ccAGTC‑GGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCgg  >  1:914858/1‑81 (MQ=255)                                                                               |                                                                            CTTCCGGCACACCACCTGCCATAAAGGCATTGACCGTTGGATGATAAACCGGGCCATTAGGCAGTACGCTCACCAGTCGGGGCACGCGCTTGTTGATGCGGATCCAGTCATCAACGGTCGGGATATGGCAACCTGCCTGGTGAGCAATTGCCGG  >  NC_000913/4522993‑4523146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑