Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	451,589	Δ1 bp	coding (22/948 nt)	cyoA ←	cytochrome bo3 ubiquinol oxidase subunit 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	451,586	0	T	.	100.0%	65.4 / NA	16	coding (25/948 nt)	cyoA	cytochrome bo3 ubiquinol oxidase subunit 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (7/9);  total (7/9)

GTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGC  >  NC_000913/451508‑451659                                                                               |                                                                          gTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACtt                                                                          >  2:99292/1‑80 (MQ=255) gTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACtt                                                                          >  1:99293/1‑80 (MQ=255)  tCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACttta                                                                        <  1:99256/81‑2 (MQ=255)  tCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACttt                                                                         <  1:99255/80‑1 (MQ=255)  tCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACt                                                                           <  2:99257/78‑1 (MQ=255)          gATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTAttt                                                               <  2:99258/81‑1 (MQ=255)               aaCAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCt                                                            >  2:99280/1‑79 (MQ=255)                 cAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGt                                                        <  1:391669/81‑1 (MQ=255)                      cAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCAt                                                   >  1:99281/1‑81 (MQ=255)                        gAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCAtt                                                  >  1:99282/1‑80 (MQ=255)                              cAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGa                                            <  1:99259/80‑1 (MQ=255)                                 ccACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTc                                        <  2:99260/81‑1 (MQ=255)                                       aGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAAtt                                    <  1:99261/79‑1 (MQ=255)                                          aaTACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACg                               <  1:99263/81‑1 (MQ=255)                                          aaTACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACg                               <  2:99262/81‑1 (MQ=255)                                               aGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATc                          <  2:99264/81‑1 (MQ=255)                                                      gCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGc                   >  2:99296/1‑81 (MQ=255)                                                        aaaTAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGt                 >  2:99287/1‑81 (MQ=255)                                                         aataatGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGtt                >  2:99288/1‑81 (MQ=255)                                                                  aaCCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAgtgtg       >  2:99299/1‑81 (MQ=255)                                                                       tCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGc  <  1:99265/81‑1 (MQ=255)                                                                               |                                                                          GTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGC  >  NC_000913/451508‑451659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑