Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	529,234	Δ1 bp	intergenic (+104/‑263)	ybbD → / → ylbI	putative uncharacterized protein YbbD/RHS element protein YlbI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	529,233	0	C	.	100.0%	38.8 / NA	10	intergenic (+103/‑264)	ybbD/ylbI	putative uncharacterized protein YbbD/RHS element protein YlbI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (8/2);  total (8/2)

CCATATCGAAGTCGTCAACTTCGTAGTGAGGAAAAGTAAAATTCCTGACTGAGAAAAGACATGTCGGCTATTGTGTAAAGCCATATAGCTCAGACGATGAATATCTACTCGTATTCAGTTGTTTATTGAGGGTGAGTTCCGACCCTGAAACAACA  >  NC_000913/529153‑529307                                                                                 |                                                                           ccATATCGAAGTCGTCAACTTCGTAGTGAGGAAAAGTAAAATTCCTGACTGAGAAAAGACATGTCGGCTATTGTGTAAAGc                                                                            <  1:115780/81‑1 (MQ=255)         aaGTCGTCAACTTCGTAGTGAGGAAAAGTAAAATTCCTGACTGAGAAAAGACATGTCGGCTATTGTGTAAAG‑CATATAGCt                                                                   >  1:115796/1‑81 (MQ=255)          aGTCGTCAACTTCGTAGTGAGGAAAAGTAAAATTCCTGACTGAGAAAAGACATGTCGGCTATTGTGTAAAG‑CATATAGCt                                                                   >  1:115803/1‑80 (MQ=255)            tcgtcAACTTCGTAGTGAGGAAAAGTAAAATTCCTGACTGAGAAAAGACATGTCGGCTATTGTGTAAAG‑CATATAGCt                                                                   >  1:115822/1‑78 (MQ=255)                              ggAAAAGTAAAATTCCTGACTGAGAAAAGACATGTCGGCTATTGTGTAAAG‑CATATAGCTCAGACGATGAATATCTACTCg                                              <  2:115781/81‑1 (MQ=255)                                  aaGTAAAATTCCTGACTGAGAAAAGACATGTCGGCTATTGTGTAAAG‑CATATAGCTCAGACGATGAATATCTACTCGTAtt                                          >  1:115801/1‑81 (MQ=255)                                   aGTAAAATTCCTGACTGAGAAAAGACATGTCGGCTATTGTGTAAAG‑CATATAGCTCAGACGATGAATATCTACTCGTATTc                                         <  1:115782/81‑1 (MQ=255)                                            ccTGACTGAGAAAAGACATGTCGGCTATTGTGTAAAG‑CATATAGCTCAGACGATGAATATCTACTCGTATTCAGTTGTTTa                                >  2:115798/1‑81 (MQ=255)                                                actgaGAAAAGACATGTCGGCTATTGTGTAAAG‑CATATAGCTCAGACGATGAATATCTACTCGTATTCAGTTGTTTAtt                              >  1:115807/1‑79 (MQ=255)                                                                        tgtgTAAAG‑CATATAGCTCAGACGATGAATATCTACTCGTATTCAGTTGTTTATTGAGGGTGAGTTCCGACCCTGAaacaa    >  2:115811/1‑81 (MQ=255)                                                                           gtAAAG‑CATATAGCTCAGACGATGAATATCTACTCGTATTCAGTTGTTTATTGAGGGTGAGTTCCGACCCTGAAacaaca  >  2:115809/1‑80 (MQ=255)                                                                                 |                                                                           CCATATCGAAGTCGTCAACTTCGTAGTGAGGAAAAGTAAAATTCCTGACTGAGAAAAGACATGTCGGCTATTGTGTAAAGCCATATAGCTCAGACGATGAATATCTACTCGTATTCAGTTGTTTATTGAGGGTGAGTTCCGACCCTGAAACAACA  >  NC_000913/529153‑529307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑