Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	549,554	Δ1 bp	coding (1198/1260 nt)	ylbE →	DUF1116 domain‑containing protein YlbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	549,552	0	G	.	100.0%	182.4 / NA	42	coding (1196/1260 nt)	ylbE	DUF1116 domain‑containing protein YlbE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (25/17);  total (25/17)

GGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCA  >  NC_000913/549476‑549626                                                                             |                                                                           ggCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTTCggcac                                                                        >  2:119773/1‑78 (MQ=255) ggCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCggcac                                                                        >  1:119756/1‑78 (MQ=255)   cATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCg                                                                     <  2:119705/81‑1 (MQ=255)        cGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGc                                                                >  1:119764/1‑81 (MQ=255)          ccACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGt                                                              <  1:119706/81‑1 (MQ=255)               catcaATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCttt                                                         >  1:119783/1‑81 (MQ=255)               catcaATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCttt                                                         >  1:119755/1‑81 (MQ=255)                atcaATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCttt                                                         >  1:119788/1‑80 (MQ=255)                atcaATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTg                                                        <  2:119707/81‑1 (MQ=255)                   aaTACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAAc                                                     <  2:119708/81‑1 (MQ=255)                    aTACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACa                                                    <  1:119709/81‑1 (MQ=255)                       ccGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGgc                                                 <  1:119710/81‑1 (MQ=255)                         ggTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGgcgc                                               >  2:119759/1‑81 (MQ=255)                         ggTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGgcgc                                               <  2:119711/81‑1 (MQ=255)                          gTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCt                                              <  2:119712/81‑1 (MQ=255)                          gTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCt                                              >  1:119780/1‑81 (MQ=255)                              cGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTgg                                            >  2:119786/1‑79 (MQ=255)                                cccATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGc                                        <  1:119713/81‑1 (MQ=255)                                     aaaGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTgg                                   >  2:119767/1‑81 (MQ=255)                                       agagGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCt                                 >  2:119754/1‑81 (MQ=255)                                        gagGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCt                                 >  1:119784/1‑80 (MQ=255)                                         agGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTg                                >  1:119776/1‑80 (MQ=255)                                         agGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGa                               <  1:119714/81‑1 (MQ=255)                                          ggCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGaa                              <  2:119715/81‑1 (MQ=255)                                           gCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGaaa                             <  1:119716/81‑1 (MQ=255)                                           gCGGGGATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGa                               >  2:119795/1‑79 (MQ=255)                                             ggggATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGc                           <  1:119717/81‑1 (MQ=255)                                                gATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATg                        >  1:119768/1‑81 (MQ=255)                                                gATCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATg                        <  1:119718/81‑1 (MQ=255)                                                  tCGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATggg                      >  1:119757/1‑81 (MQ=255)                                                   cGGGCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATggg                      >  2:119771/1‑80 (MQ=255)                                                     ggCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTAt                   <  2:119720/81‑1 (MQ=255)                                                     ggCAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTAt                   >  1:119774/1‑81 (MQ=255)                                                       cAGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTg                 >  1:119787/1‑81 (MQ=255)                                                        aGATTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTgg                >  1:119779/1‑81 (MQ=255)                                                          aTTGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGtt              >  1:119761/1‑81 (MQ=255)                                                           ttGGCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGtt              >  1:119797/1‑80 (MQ=255)                                                             ggCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAg           >  1:119766/1‑81 (MQ=255)                                                             ggCGCAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAg           >  2:119791/1‑81 (MQ=255)                                                                 cAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAc       >  1:119782/1‑81 (MQ=255)                                                                 cAGGCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAc       >  2:119777/1‑81 (MQ=255)                                                                   ggCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAcgc     <  1:119721/81‑1 (MQ=255)                                                                   ggCACCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAcgc     >  2:119790/1‑81 (MQ=255)                                                                      aCCGTGC‑GGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCa  <  2:119722/81‑1 (MQ=255)                                                                             |                                                                           GGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCA  >  NC_000913/549476‑549626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑