Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	584,262	(A)7→6	coding (582/750 nt)	appY →	DNA‑binding transcriptional activator AppY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	584,256	0	A	.	100.0%	33.2 / NA	9	coding (576/750 nt)	appY	DNA‑binding transcriptional activator AppY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (5/4);  total (5/4)

AAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCA  >  NC_000913/584193‑584328                                                                |                                                                         aaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGC‑AAAAAACTCATAACTTc                                                         >  1:125693/1‑80 (MQ=255)      tAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGC‑AAAAAACTCATAACTTCAAActc                                                   >  2:125691/1‑81 (MQ=255)      tAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGC‑AAAAAACTCATAACTTCAAActc                                                   >  2:125694/1‑81 (MQ=255)                   aaCGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGC‑AAAAAACTCATAACTTCAAACTCTTATTCTATCAAt                                      >  1:125696/1‑81 (MQ=255)                      gTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGC‑AAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTc                                   <  1:125673/81‑1 (MQ=255)                      gTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGC‑AAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTc                                   <  1:125674/81‑1 (MQ=255)                                 aaaTATTGAGAGATACTAGGATGAGGTATGC‑AAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGaa                        <  2:125676/81‑1 (MQ=255)                                 aaaTATTGAGAGATACTAGGATGAGGTATGC‑AAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGa                         <  1:125675/80‑1 (MQ=255)                                                       gAGGTATGC‑AAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttca  >  2:125698/1‑81 (MQ=255)                                                                |                                                                         AAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCA  >  NC_000913/584193‑584328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑