Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	681,557	(A)7→6	coding (1346/1452 nt)	djlC →	co‑chaperone DjlC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	681,551	0	A	.	100.0%	88.3 / NA	21	coding (1340/1452 nt)	djlC	co‑chaperone DjlC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (8/13);  total (8/13)

CCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAG  >  NC_000913/681473‑681625                                                                               |                                                                           cccGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGaaa                                                                          <  2:144754/81‑1 (MQ=255)  ccGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGaaaa                                                                         <  2:144755/81‑1 (MQ=255)    ggATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGaaaaaa                                                                       >  1:144795/1‑81 (MQ=255)        gCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGaaaaaatgat                                                                   >  2:144789/1‑77 (MQ=255)         cTTTTGACGAAAGTGAAGCTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGaaaaaatgat                                                                   <  1:144757/80‑5 (MQ=255)         cTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAatgatg                                                                 <  1:144756/81‑1 (MQ=255)                 gAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGaa                                                         <  1:144758/81‑1 (MQ=255)                 gAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGa                                                          >  1:144799/1‑80 (MQ=255)                       gAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACca                                                   >  2:144793/1‑81 (MQ=255)                        aaaCTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACca                                                   <  1:144759/80‑1 (MQ=255)                           cTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACaga                                               <  1:144760/81‑1 (MQ=255)                              gAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGc                                             <  2:144761/80‑1 (MQ=255)                              gAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCa                                            >  2:144802/1‑81 (MQ=255)                                 ccGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCATTa                                         <  2:144762/81‑1 (MQ=255)                                    gaggaTGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCa                                      <  2:144763/81‑1 (MQ=255)                                         tGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCAtt                                 <  1:144764/81‑1 (MQ=255)                                           aaGATGTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTa                                <  1:144765/80‑1 (MQ=255)                                           aaGATGTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTAt                               <  2:144766/81‑1 (MQ=255)                                                gTCGATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCaaaa                          >  1:144792/1‑81 (MQ=255)                                                   gATGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAAt                         >  1:144796/1‑79 (MQ=255)                                                    aTGAGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAg                      >  2:144794/1‑81 (MQ=255)                                                       aGCCAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGttt                   <  1:144767/81‑1 (MQ=255)                                                          cAACACCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAAc                <  1:144768/81‑1 (MQ=255)                                                             cacCTGAAAATCATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGt             <  1:144769/81‑1 (MQ=255)                                                                        cATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAg  >  2:144803/1‑81 (MQ=255)                                                                        cATGAAG‑AAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAg  >  2:144810/1‑81 (MQ=255)                                                                               |                                                                           CCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAG  >  NC_000913/681473‑681625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑