Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	856,129	Δ1 bp	coding (167/1593 nt)	ybiT →	ABC transporter ATP‑binding protein YbiT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	856,127	0	G	.	100.0%	87.8 / NA	21	coding (165/1593 nt)	ybiT	ABC transporter ATP‑binding protein YbiT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (8/13);  total (8/13)

ACGGCCTGATTGGCGCGAACGGTAGTGGTAAATCCACCTTTATGAAGATCCTCGGCGGCGACCTTGAGCCGACGCTGGGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACTGCGTCAGGATCAGTTTGCCTTTGAAGAGTTCAC  >  NC_000913/856051‑856201                                                                             |                                                                           aCGGCCTGATTGGCGCGAACGGTAGTGGTAAATCCACCTTTATGAAGATCCTCGGCGGCGACCTTGAGCCGACGCTggtaa                                                                        >  1:178667/1‑78 (MQ=255)        gATTGGCGCGAACGGTAGTGGTAAATCCACCTTTATGAAGATCCTCGGCGGCGACCTTGAGCCGACGCT‑GGTAACGTTTcc                                                                >  1:178645/1‑81 (MQ=255)           tGGCGCGAACGGTAGTGGTAAATCCACCTTTATGAAGATCCTCGGCGGCGACCTTGAGCCGACGCT‑GGTAACGTTTCCCTc                                                             >  2:178669/1‑81 (MQ=255)             gcgcGAACGGTAGTGGTAAATCCACCTTTATGAAGATCCTCGGCGGCGACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCg                                                            <  2:178612/80‑1 (MQ=255)                   aCGGTAGTGGTAAATCCACCTTTATGAAGATCCTCGGCGGCGACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCaa                                                     <  1:178613/81‑1 (MQ=255)                       tAGTGGTAAATCCACCTTTATGAAGATCCTCGGCGGCGACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAg                                                 >  2:178664/1‑81 (MQ=255)                               aaTCCACCTTTATGAAGATCCTCGGCGGCGACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTgg                                         >  2:178652/1‑81 (MQ=255)                               aaTCCACCTTTATGAAGATCCTCGGCGGCGACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTg                                          <  1:178614/80‑1 (MQ=255)                                    aCCTTTATGAAGATCCTCGGCGGCGACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAAc                                    <  2:178615/81‑1 (MQ=255)                                     ccTTTATGAAGATCCTCGGCGGCGACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACt                                   <  1:178616/81‑1 (MQ=255)                                             aaGATCCTCGGCGGCGACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACTGCGTCAgg                           <  1:178617/81‑1 (MQ=255)                                                aTCCTCGGCGGCGACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACTGCGTCAGGATc                        <  2:178618/81‑1 (MQ=255)                                                      ggcggcGACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACTGCGTCAGGATCAGttt                   <  1:178619/80‑1 (MQ=255)                                                         ggcgACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACTGCGTCAGGATCAGTTTGCCt               >  1:178671/1‑81 (MQ=255)                                                         ggcgACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACTGCGTCAGGATCAGTTTGCCt               >  2:178661/1‑81 (MQ=255)                                                         ggcgACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACTGCGTCAGGATCAGTTTGCCt               >  1:178658/1‑81 (MQ=255)                                                         ggcgACCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACTGCGTCAGGATCAGTTTGCCt               >  1:178644/1‑81 (MQ=255)                                                             aCCTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACTGCGTCAGGATCAGTTTGCCTTTGa           <  2:178620/81‑1 (MQ=255)                                                               cTTGAGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACTGCGTCAGGATCAGTTTGCCTTTGAag         <  2:178621/81‑1 (MQ=255)                                                                   aGCCGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACTGCGTCAGGATCAGTTTGCCTTTGAAGAGtt     <  1:178622/81‑1 (MQ=255)                                                                     ccGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGT‑AACTGCGTCAGGATCAGTTTGCCTTTGAAGAGTTCAc  <  2:628233/81‑1 (MQ=255)                                                                      cGACGCT‑GGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACTGCGTCAGGATCAGTTTGCCTTTGAAGAGTTCAc  <  1:178623/81‑1 (MQ=255)                                                                             |                                                                           ACGGCCTGATTGGCGCGAACGGTAGTGGTAAATCCACCTTTATGAAGATCCTCGGCGGCGACCTTGAGCCGACGCTGGGTAACGTTTCCCTCGATCCCAACGAGCGCATTGGTAAACTGCGTCAGGATCAGTTTGCCTTTGAAGAGTTCAC  >  NC_000913/856051‑856201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑