| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,134,618 | (G)5→6 | intergenic (+61/‑111) | flgF → / → flgG | flagellar basal‑body rod protein FlgF/flagellar basal‑body rod protein FlgG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,134,613 | 1 | . | G | 93.8% | 48.0 / ‑2.8 | 16 | intergenic (+56/‑116) | flgF/flgG | flagellar basal‑body rod protein FlgF/flagellar basal‑body rod protein FlgG |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (9/6); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
CAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTAT‑GGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAG > NC_000913/1134533‑1134686 | caaccaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTAt < 1:226950/81‑1 (MQ=255) accaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTAT‑gg < 1:226951/81‑1 (MQ=255) tCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGGCAATc < 1:226952/79‑1 (MQ=255) gAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTAT‑GGGGGCAATCGCAATTTa > 1:226978/1‑81 (MQ=255) aaTTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGGCAATCGCAATTTATCGa > 1:226972/1‑81 (MQ=255) aaaGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGGCAATCGCAATTTATCGAtttt < 2:226953/80‑1 (MQ=255) aaaGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGGCAATCGCAATTTATCGATTTTg > 2:226986/1‑81 (MQ=255) aaaGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGGCAATCGCAATTTATCGATTTTg > 2:226983/1‑81 (MQ=255) aTGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGGCAATCGCAATTTATCGATTTTGCGAGCACtt > 2:226981/1‑81 (MQ=255) aCAAGTATAAGTTGCTCGATGCGCAAGTTTATCGGGTCTATGGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTg > 2:226984/1‑79 (MQ=255) cAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAg > 1:226979/1‑81 (MQ=255) taAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCgg < 1:226954/80‑1 (MQ=255) gTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGAt > 1:226994/1‑79 (MQ=255) aTGCGCAAGTTTATCGGGTCTATGGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTa < 2:226955/81‑1 (MQ=255) gcAAGTTTATCGGGTCTATGGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgcc < 2:226956/81‑1 (MQ=255) cAAGTTTATCGGGTCTATGGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgccg < 1:226957/81‑1 (MQ=255) aTCGGGTCTATGGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGCAGGCCGGATAAGGCGTTTACGCCGCATCCgg > 2:226991/1‑81 (MQ=25) gTCTATGGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaag > 1:226985/1‑80 (MQ=255) | CAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTAT‑GGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAG > NC_000913/1134533‑1134686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |