| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,392,761 | (A)8→9 | coding (771/900 nt) | pgrR → | DNA‑binding transcriptional repressor PgrR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,392,753 | 1 | . | A | 92.3% | 39.3 / ‑2.7 | 13 | coding (763/900 nt) | pgrR | DNA‑binding transcriptional repressor PgrR |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (4/8); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GACCTGATCATTGATGCTGCAATTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑AAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTACCCGGTTATCACCTGTACTATCCACACC > NC_000913/1392675‑1392825 | gACCTGATCATTGATGCTGCAATTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aa < 1:265983/81‑1 (MQ=255) tGATCATTGATGCTGCAATTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaaa > 1:266001/1‑80 (MQ=255) aTTGATGCTGCAATTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaaaaaaact > 2:265998/1‑78 (MQ=255) ttGATGCTGCAATTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑aaaaaaaaactg < 2:265984/81‑5 (MQ=255) ctgcAATTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑AAAAAAAACTGATACgt < 2:265985/80‑1 (MQ=255) cAATTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACgtgt < 2:265986/80‑1 (MQ=255) ggCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATaaa < 2:265987/81‑1 (MQ=255) aTTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCacac < 1:265988/81‑1 (MQ=255) aTTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCacac > 2:266006/1‑81 (MQ=255) ggCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCacac > 1:266003/1‑78 (MQ=255) ccTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTAcc < 2:265989/78‑1 (MQ=255) ccTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTACCCg < 1:265990/80‑1 (MQ=255) cTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTACCCGGt < 2:265991/81‑1 (MQ=255) aTCAGGTTGAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTACCCGGTTATCAc > 2:266000/1‑81 (MQ=255) gAGCGGGCTATTAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTACCCGGTTATCACCTGTACTa > 2:266014/1‑81 (MQ=255) ttAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTACCCGGTTATCACCTGTACTATCCACAcc < 2:265992/79‑1 (MQ=255) ttAAAGAAAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTACCCGGTTATCACCTGTACTATCCACAcc < 1:265993/79‑1 (MQ=255) | GACCTGATCATTGATGCTGCAATTGATGGGCATGGATTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAG‑AAAAAAAACTGATACGTGTTTTGGATAAATTCACACCAGATTTACCCGGTTATCACCTGTACTATCCACACC > NC_000913/1392675‑1392825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |