Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,417,546 (T)8→9 coding (218/276 nt) racC ← Rac prophage; protein RacC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,417,5381.T91.7% 35.6 / ‑2.7 12coding (226/276 nt)racCRac prophage; protein RacC
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (8/3);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

ATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGT  >  NC_000913/1417470‑1417605
                                                                     |                                                                   
aTACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑tttttttttctg                                                         >  1:269505/1‑77 (MQ=255)
      aGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTTCTGCGGGCa                                                    <  1:269490/81‑1 (MQ=255)
        gCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTTCTGCggg                                                      >  1:269503/1‑77 (MQ=255)
         cTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTTCTGCGGGCAGAt                                                 >  1:269508/1‑81 (MQ=255)
                   cAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGat                                       <  1:269491/80‑1 (MQ=255)
                         tCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCAc                                 >  2:269510/1‑81 (MQ=255)
                                       cATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAtttttt                   >  1:269514/1‑81 (MQ=255)
                                        aTTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAttttttt                  >  1:269511/1‑81 (MQ=255)
                                             aaTTAGAGTTGCCAGAAATTCGGCTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGacac             <  1:269492/81‑1 (MQ=255)
                                                tAGAGTTGCCAGAAATTCGGCTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACGtt          >  2:269509/1‑81 (MQ=255)
                                                   agTTGCCAGAAATTCGGCTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTtcat       >  1:269517/1‑81 (MQ=255)
                                                       gCCAGAAATTCGGCTTTTTTTTTCTTCGGGCAGTTTCTTTCCGATATTCTCCTTGCACTTTTTTTTTACACCTTCATCaat   >  1:269512/1‑80 (MQ=255)
                                                        ccAGAAATTCGGCTTTTTTTTTCTGCGGGCAGATTCTTTCCGAAATGCACCAGGCACATTTTTTTGACACCTTCATCAAgt  <  2:269493/81‑1 (MQ=255)
                                                                     |                                                                   
ATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGT  >  NC_000913/1417470‑1417605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑