Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,894,328	(A)6→7	coding (196/300 nt)	yoaC →	DUF1889 domain‑containing protein YoaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,894,322	1	.	A	100.0%	23.7 / NA	8	E64K (GAA→AAA)	yoaC	DUF1889 domain‑containing protein YoaC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (2/6);  total (2/6)

AAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGG‑AAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTT  >  NC_000913/1894243‑1894392                                                                                 |                                                                       aaaaGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACgg                                                                         >  2:333999/1‑80 (MQ=255)          gggAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGG‑aaaaaaatgg                                                              >  2:334001/1‑77 (MQ=255)                 cccGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAAATGGTTGGAt                                                         <  1:333981/80‑1 (MQ=255)                                 atatTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAAATGGTTGGATGGGCAAAAAAAATGgag                                        <  1:333982/81‑1 (MQ=255)                                  tatTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAAATGGTTGGATGGGCAAAAAAAATGgaga                                       <  1:333983/81‑1 (MQ=255)                                                   aCCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGTTCtgtg                      <  1:333985/81‑1 (MQ=255)                                                   aCCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGTTCtg                        <  1:333984/79‑1 (MQ=255)                                                        gAAGGCTGGAACCCTGGGTTCACGGAAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTaa                 <  1:333986/81‑1 (MQ=255)                                                                gAACCCTGGGTTCACGGAAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCt          >  1:333996/1‑80 (MQ=255)                                                                       gggTTCACGGAAAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACtt  >  1:334006/1‑81 (MQ=255)                                                                                 |                                                                       AAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGG‑AAAAAATGGTTGGATGGGCAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTT  >  NC_000913/1894243‑1894392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑