Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,107,972	(T)8→10	coding (358/1104 nt)	glf ←	UDP‑galactopyranose mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,107,964	1	.	T	100.0%	31.6 / NA	10	K122K (AAG→AAA)	glf	UDP‑galactopyranose mutase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (4/6);  total (4/6)
*	NC_000913	2,107,964	2	.	T	100.0%	31.9 / NA	10	K122K (AAG→AAA)	glf	UDP‑galactopyranose mutase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (4/6);  total (4/6)

ATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATAC‑‑TTTTTTTTCTGAGCATTAATGATATTTTGAGCTTCTTGAGGATCTTTAACTCCCCACATTT  >  NC_000913/2107887‑2108025                                                                               ||                                                              aTCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATAC‑‑tt                                                             <  2:367857/80‑1 (MQ=255)    aaTGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATAC‑‑tttttt                                                         <  1:367859/81‑1 (MQ=255)    aaTGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATAC‑‑ttttt                                                          <  1:367858/80‑1 (MQ=255)          tGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATAC‑‑ttttttttttct                                                   >  1:367880/1‑77 (MQ=255)           ggTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATAC‑‑ttttttttttct                                                   >  2:367883/1‑76 (MQ=255)                     ctccCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTTTCTGAGCATTAAt                                           <  2:367861/80‑1 (MQ=255)                            aCTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTTTCTGAGCATTAATGATAtttt                                   >  2:367886/1‑81 (MQ=255)                                    aaTCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTTTCTGAGCATTAATGATATTTTGAGcttct                           >  1:367885/1‑81 (MQ=255)                                      tCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTTTCTGAGCATTAATGATATTTTGAGCTTCTTg                         <  2:367862/81‑1 (MQ=255)                                         ccTGCTCCTCCAAATTTTCAGGTACCTTGTCGCCATACTTTTTTTTTTCTGAGCATTAATGATATTTTGAGCTTCTTGAgg                      <  2:367863/81‑1 (MQ=255)                                               cctccAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTTTCTGAGCATTAATGATATTTTGAGCTTCTTGAGGATCtt                 >  1:367882/1‑80 (MQ=255)                                                ctccAAATTTTCAGGTACCTTGTCACCATACTTTTTTTTTTCTGAGCATTAATGATATTTTGAGCTTCTTGAGGATCttt                >  2:367888/1‑80 (MQ=255)                                                      aTTTTCAGGTACCTTGTCACCATACTTTTTTTTTTCTGAGCATTAATGATATTTTGAGCTTCTTGAGGATCTTTAACTccc         <  1:367864/81‑1 (MQ=255)                                                         ttCAGGTACCTTGTCACCATACTTTTTTTTTTCTGAGCATTAATGATATTTTGAGCTTCTTGAGGATCTTTAACTCCCca       <  2:367865/80‑1 (MQ=255)                                                             ggTACCTTGTCACCATACTTTTTTTTTTCTGAGCATTAATGATATTTTGAGCTTCTTGAGGATCTTTAACTCCCCACAttt  <  2:367866/81‑1 (MQ=255)                                                                               ||                                                              ATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGTACCTTGTCACCATAC‑‑TTTTTTTTCTGAGCATTAATGATATTTTGAGCTTCTTGAGGATCTTTAACTCCCCACATTT  >  NC_000913/2107887‑2108025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑