| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,355,314 | (T)7→8 | coding (1050/1260 nt) | glpB → | anaerobic glycerol‑3‑phosphate dehydrogenase subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,355,307 | 1 | . | T | 96.9% | 112.1 / ‑3.1 | 32 | coding (1043/1260 nt) | glpB | anaerobic glycerol‑3‑phosphate dehydrogenase subunit B |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (21/10); total (22/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGA‑TTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCA > NC_000913/2355232‑2355382 | gTAACGGCATTCGAGGGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGA‑ttttt < 2:413167/81‑1 (MQ=255)gTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGA‑ttttt > 2:413236/1‑81 (MQ=255) ggCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGA‑ttttttttgc > 1:413221/1‑78 (MQ=255) ggCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGA‑ttttttttgc > 1:413234/1‑78 (MQ=255) cGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGA‑TTTTTTTGCGCCGCaa > 2:413241/1‑81 (MQ=255) gagaGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCaa > 2:413232/1‑81 (MQ=255) gagaGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCaa > 2:413215/1‑81 (MQ=255) ccGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTg > 2:413229/1‑81 (MQ=255) ccGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTg < 2:413168/81‑1 (MQ=255) cGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTgg > 2:413223/1‑81 (MQ=255) cGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTgg < 2:413169/81‑1 (MQ=255) gATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGgc < 2:413170/81‑1 (MQ=255) aTTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTgg > 2:413219/1‑79 (MQ=255) aTTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGgca < 1:413171/81‑1 (MQ=255) ttCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGgcag < 2:413172/81‑1 (MQ=255) tctcGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGgcag > 1:413237/1‑80 (MQ=255) ctcGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGgcagc < 2:413173/80‑1 (MQ=255) gCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTc > 1:413191/1‑81 (MQ=255) tGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGgtg > 2:413220/1‑81 (MQ=255) aTGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGgtgt > 2:413233/1‑80 (MQ=255) gCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCa > 2:413226/1‑81 (MQ=255) aCAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACt > 1:413216/1‑80 (MQ=255) aCCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACtgat > 1:413231/1‑79 (MQ=255) ccGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACtgat > 1:413225/1‑78 (MQ=255) cGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATgaga > 1:413242/1‑81 (MQ=255) gCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGAc > 2:413222/1‑81 (MQ=255) gggTGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGcc < 2:413174/81‑1 (MQ=255) tGAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGt > 1:413240/1‑81 (MQ=255) gAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGt > 1:413230/1‑80 (MQ=255) gAATGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGt > 1:413228/1‑80 (MQ=255) aaTGGTATAAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTca > 2:413247/1‑81 (MQ=255) taAGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCAcaggcag > 1:413246/1‑81 (MQ=255) aGGGAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAggg < 1:413175/81‑1 (MQ=255) gggAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAggg > 2:413244/1‑80 (MQ=255) gggAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGc < 1:413176/81‑1 (MQ=255) ggAGATTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCa < 2:413177/81‑1 (MQ=255) | GTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGA‑TTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCA > NC_000913/2355232‑2355382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |