Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,460,058	(T)6→7	coding (412/1311 nt)	fadI ←	3‑ketoacyl‑CoA thiolase FadI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,460,052	1	.	T	100.0%	124.8 / NA	35	L140M (CTG→ATG)	fadI	3‑ketoacyl‑CoA thiolase FadI
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (15/20);  total (15/20)

GCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAG‑TTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGC  >  NC_000913/2459972‑2460127                                                                                  |                                                                            gcgcAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAg                                                                              <  2:433461/81‑1 (MQ=255)  cgcAAACGCAGGCGAGAGAAGAGTTTCAGTCACTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAG‑t                                                                            <  2:433462/81‑1 (MQ=255)   gcAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAG‑tt                                                                           <  1:433463/81‑1 (MQ=255)           aGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAG‑tttttttact                                                                   <  1:433464/81‑5 (MQ=255)              cGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGAc                                                                 >  1:433510/1‑81 (MQ=255)               gagagaAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACg                                                                >  1:433513/1‑81 (MQ=255)               gagagaAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACg                                                                <  2:433465/81‑1 (MQ=255)                 gagaAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGcc                                                              <  1:903918/81‑1 (MQ=255)                 gagaAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGcc                                                              <  2:433466/81‑1 (MQ=255)                   gaagaGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCaa                                                            >  1:433514/1‑81 (MQ=255)                       agTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTgg                                                        <  1:433467/81‑1 (MQ=255)                            cAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAAt                                                    <  1:433468/80‑1 (MQ=255)                            cAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACACGCGCCAGTTTTTTTACTGACGCCAATTGGCAAt                                                    <  2:433469/80‑1 (MQ=255)                             aGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATAc                                                  <  2:433470/81‑1 (MQ=255)                              gTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATAcc                                                 <  2:433471/81‑1 (MQ=255)                              gTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATAcc                                                 <  2:433472/81‑1 (MQ=255)                              gTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATAcc                                                 <  1:433473/81‑1 (MQ=255)                                cGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCg                                                >  2:433533/1‑80 (MQ=255)                                 gCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCg                                                >  2:433511/1‑79 (MQ=255)                                   tGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGaa                                              >  1:433529/1‑79 (MQ=255)                                     gCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAagag                                           >  1:433509/1‑80 (MQ=255)                                      cTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAgg                                          >  1:433517/1‑80 (MQ=255)                                      cTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGa                                         <  1:433474/81‑1 (MQ=255)                                         aTGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAAt                                       <  1:433475/80‑1 (MQ=255)                                         aTGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAAt                                       <  2:433476/80‑1 (MQ=255)                                          tGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCt                                     <  1:433477/81‑1 (MQ=255)                                              aCGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGccc                                 >  2:433523/1‑81 (MQ=255)                                                    tttGTTGACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCgg                           >  1:433528/1‑81 (MQ=255)                                                      tgttgACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCgg                           <  2:433478/79‑1 (MQ=255)                                                      tgttgACATCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGc                          >  1:433535/1‑80 (MQ=255)                                                             aTCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCg                  >  1:433531/1‑81 (MQ=255)                                                              tCAACCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGc                 <  1:433479/81‑1 (MQ=255)                                                                aaCCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCt                <  1:433480/80‑1 (MQ=255)                                                                 aCCAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCg              <  1:433481/81‑1 (MQ=255)                                                                   cAGCACGCGCGCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGaa            >  1:433526/1‑81 (MQ=255)                                                                        cgcgcgCCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCTGCAATCCCCGCTCGAATAGtt       <  2:433482/81‑1 (MQ=255)                                                                           gcgcCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTccc    <  2:433483/81‑1 (MQ=255)                                                                             gcCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGc  >  1:433525/1‑81 (MQ=255)                                                                             gcCAGTTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGc  >  2:433516/1‑81 (MQ=255)                                                                                  |                                                                            GCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAG‑TTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGC  >  NC_000913/2459972‑2460127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑