Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,677,262	(T)6→7	coding (1100/1512 nt)	yphE ←	putative ABC transporter ATP‑binding protein YphE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,677,256	1	.	T	100.0%	92.6 / NA	26	I369N (ATC→AAC)	yphE	putative ABC transporter ATP‑binding protein YphE
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (11/15);  total (11/15)

CGGCCTTGACCGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGA‑TTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGATCCCCGCTTCTTTGCGGTTTT  >  NC_000913/2677176‑2677331                                                                                  |                                                                            cggcCTTGACCGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGa                                                                              <  1:477111/81‑1 (MQ=255)     cTTGACCGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGA‑tttt                                                                         >  2:477166/1‑81 (MQ=255)       tGACCGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGA‑tttttt                                                                       >  2:477151/1‑81 (MQ=255)          ccGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGA‑tttttttgc                                                                    <  2:477112/81‑4 (MQ=255)                tCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTg                                                               <  1:477114/81‑1 (MQ=255)                  cGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACAGTTGGCGCTGATTTTTTTGCCGATTGGt                                                             <  2:477115/81‑1 (MQ=255)                        aTCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCAc                                                       >  2:477174/1‑81 (MQ=255)                           aCCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGt                                                    <  1:477116/81‑1 (MQ=255)                                  cGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTAtttt                                               <  1:477117/79‑1 (MQ=255)                                  cGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTAttt                                                <  2:477118/78‑1 (MQ=255)                                    gTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGt                                            >  2:477165/1‑80 (MQ=255)                                       aGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCaa                                         <  2:477119/80‑1 (MQ=255)                                         gcggcgGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGc                                      <  2:477120/81‑1 (MQ=255)                                          cggcggATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGcc                                     <  2:477121/81‑1 (MQ=255)                                           ggcggATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGcc                                     >  2:477164/1‑80 (MQ=255)                                                  ggtggACCATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAgg                             >  1:477177/1‑81 (MQ=255)                                                   gtggACCATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAggg                            <  2:477123/81‑1 (MQ=255)                                                   gtggACCATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAggg                            <  1:477122/81‑1 (MQ=255)                                                         cATTGCAGCACACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGAt                      >  1:477173/1‑81 (MQ=255)                                                               agcaCACCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGATCCCCGc                <  2:477124/81‑1 (MQ=255)                                                                   cacCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGATCCCCGcttct            <  2:477125/81‑1 (MQ=255)                                                                   cacCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGATCCCCGcttct            >  2:477185/1‑81 (MQ=255)                                                                    acCGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGATCCCCGcttctt           >  2:477216/1‑81 (MQ=255)                                                                     ccGTTGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGATCCCCGCTTCttt          <  2:477126/81‑1 (MQ=255)                                                                        ttGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGATCCCCGCTTCTTTGCg       <  2:477127/81‑1 (MQ=255)                                                                         tGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGATCCCCGCTTCTTTGCgg      >  1:477188/1‑81 (MQ=255)                                                                         tGGCGCTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGATCCCCGCTTCTTTGCgg      >  1:477168/1‑81 (MQ=255)                                                                           gcgcTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGATCCCCGCTTCTTTGCGGtt    <  2:477128/81‑1 (MQ=255)                                                                             gcTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGATCCCCGCTTCTTTGCGGtttt  >  1:477183/1‑81 (MQ=255)                                                                             gcTGATTTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGATCCCCGCTTCTTTGCGGtttt  >  2:477169/1‑81 (MQ=255)                                                                                  |                                                                            CGGCCTTGACCGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGA‑TTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAGGGAATGATCCCCGCTTCTTTGCGGTTTT  >  NC_000913/2677176‑2677331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑