| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,989,488 | (A)8→9 | coding (185/483 nt) | yqeJ → | protein YqeJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,989,480 | 1 | . | A | 91.7% | 35.6 / ‑2.7 | 12 | coding (177/483 nt) | yqeJ | protein YqeJ |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (4/7); total (4/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAG > NC_000913/2989409‑2989542 | aCATGCACTGCAAATTTGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑‑AAAAAAAAt < 2:543212/81‑1 (MQ=255) cTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACA‑AAAAAAAATAAAACa < 1:543213/81‑1 (MQ=255) gCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACA‑AAAAAAAATAAAACAg > 2:543231/1‑80 (MQ=255) ttGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACA‑AAAAAAAATAAAACAGGAACTg > 2:558598/1‑81 (MQ=255) tttttAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACA‑AAAAAAAATAAAACAGGAACTGTTGCGTTg < 2:543214/81‑1 (MQ=255) tttttAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACA‑AAAAAAAATAAAACAGGAACTGTTGCGTTg < 2:543215/81‑1 (MQ=255) aTGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACA‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTgg > 1:543244/1‑80 (MQ=255) aTGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACA‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTgg < 2:543216/80‑1 (MQ=255) aCCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACA‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAAc < 1:543217/80‑1 (MQ=255) ccAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACA‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCt < 2:543218/81‑1 (MQ=255) gAGCAAATCTTACCATCGACTTTATGTATAACA‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGcaacaa < 2:543219/81‑1 (MQ=255) tCGACTTTATGTATAACAAAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAg > 1:543239/1‑81 (MQ=255) | ACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAG > NC_000913/2989409‑2989542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |