Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,010,255	(T)7→8	coding (228/1386 nt)	hyuA →	phenylhydantoinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,010,248	1	.	T	100.0%	74.0 / NA	21	D74V (GAT→GTT)	hyuA	phenylhydantoinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (14/7);  total (14/7)

GGCTGTTACGTTTTCCCTGGCGGCGTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGA‑TTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGCGGTACAACAACCATTATTGACCATATGGGATTTG  >  NC_000913/3010169‑3010316                                                                                 |                                                                     ggCTGTTACGTTTTCCCTGGCGGCGTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTgatga‑t                                                                     >  1:546877/1‑81 (MQ=255)  gCTGTTACGTTTTCCCTGGCGGCGTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGA‑tt                                                                    <  2:546829/81‑1 (MQ=255)       tACGTTTTCCCTGGCGGCGTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGA‑ttttttt                                                               >  2:546868/1‑81 (MQ=255)           ttttCCCTGGCGGCGTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGA‑ttttttttacc                                                           >  2:546892/1‑77 (MQ=255)           ttttCCCTGGCGGCGTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGA‑ttttttttacc                                                           >  1:546881/1‑77 (MQ=255)               cccTGGCGGCGTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTa                                                        >  1:546880/1‑81 (MQ=255)                 cTGGCGGCGTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTAcc                                                      <  2:546830/81‑1 (MQ=255)                   ggcggcGTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCcg                                                    >  1:546884/1‑81 (MQ=255)                      ggcgTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCcgcg                                                  >  2:546918/1‑80 (MQ=255)                      ggcgTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCgcgc                                                 <  2:546831/81‑1 (MQ=255)                         gTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGc                                              >  2:546886/1‑81 (MQ=255)                            gATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGc                                           >  1:546897/1‑81 (MQ=255)                            gATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGc                                           >  1:546875/1‑81 (MQ=255)                             aTGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGCg                                          >  2:546879/1‑81 (MQ=255)                                   aTACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGc                                    >  1:546893/1‑81 (MQ=255)                                        cATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGCGGTa                                >  1:546870/1‑80 (MQ=255)                                          tttCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGCGGTacaa                             <  1:546832/81‑1 (MQ=255)                                              aaTATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGCGGTACaacaac                         <  1:546834/81‑1 (MQ=255)                                              aaTATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGCGGTACaacaac                         <  1:546833/81‑1 (MQ=255)                                                     aTGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGCGGTACAACAACCattat                   <  2:546835/80‑1 (MQ=255)                                                        tCGGCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGCGGTACAACAACCATTATTGAc               >  2:546891/1‑81 (MQ=255)                                                          ggCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGCGGTACAACAACCATTATTGACCa             >  1:546895/1‑81 (MQ=255)                                                          ggCATCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGCGGTACAACAACCATTATTGACCa             >  2:546890/1‑81 (MQ=255)                                                             aTCGCGCGCAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGCGGTACAACAACCATTATTGACCatat          <  2:546836/81‑1 (MQ=255)                                                                gcgcgcAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGCGGTACAACAACCATTATTGACCATATgg        >  2:546887/1‑80 (MQ=255)                                                                     cAGTTGTGATGATTTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGCGGTACAACAACCATTATTGACCATATGGGATTTg  >  1:546889/1‑81 (MQ=255)                                                                                 |                                                                     GGCTGTTACGTTTTCCCTGGCGGCGTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGA‑TTTTTTTACCGGTACCCGCGCAGCTGCGTGTGGCGGTACAACAACCATTATTGACCATATGGGATTTG  >  NC_000913/3010169‑3010316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑