Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	397,561	(A)5→6	coding (923/1221 nt)	sbmA →	peptide antibiotic/peptide nucleic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	397,556	1	.	A	100.0%	84.5 / NA	24	R306R (CGG→CGA)	sbmA	peptide antibiotic/peptide nucleic acid transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (6/18);  total (6/18)

AGCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGG‑AAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAAC  >  NC_000913/397483‑397631                                                                           |                                                                            agCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGG‑aaaaaac                                                                      >  2:87768/1‑79 (MQ=255) agCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGG‑aaaaaac                                                                      <  2:87724/81‑3 (MQ=255)  gCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGG‑aaaaaact                                                                     <  1:87725/81‑4 (MQ=255)  gCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGG‑aaaaaact                                                                     <  2:87726/81‑4 (MQ=255)  gCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGG‑aaaaaac                                                                      >  1:87779/1‑78 (MQ=255)      gTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTAtt                                                                   <  1:87727/80‑1 (MQ=255)         tATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTcc                                                                <  1:87728/80‑1 (MQ=255)         tATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTcc                                                                <  2:87729/80‑1 (MQ=255)             gTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCctc                                                           <  2:87730/81‑1 (MQ=255)              tGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCctc                                                           >  2:87769/1‑80 (MQ=255)                  gACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTAttt                                                      <  1:87731/81‑1 (MQ=255)                  gACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTAttt                                                      >  2:87771/1‑81 (MQ=255)                   aCGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTAtttt                                                     <  1:87732/81‑1 (MQ=255)                      aTGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTATTTTCAc                                                  <  1:87734/81‑1 (MQ=255)                      aTGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTATTTTCAc                                                  <  2:87733/81‑1 (MQ=255)                         ccACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTATTTTCACtat                                               <  1:87736/81‑1 (MQ=255)                         ccACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTATTTTCACta                                                <  2:87735/80‑1 (MQ=255)                            cgcgcgcgACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTATTTTCACTATATg                                            <  2:87738/81‑1 (MQ=255)                            cgcgcgcgACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTATTTTCACTATATg                                            <  2:87737/81‑1 (MQ=255)                               gcgcgACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTATTTTCACTATATGTAt                                         >  1:87770/1‑81 (MQ=255)                                    aCGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCaa                                    <  1:87739/81‑1 (MQ=255)                                        cgccTACGGTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATc                                <  1:87740/81‑1 (MQ=255)                                                gTACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCAt                        <  1:87741/81‑1 (MQ=255)                                                 tACGCGAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCAtt                       >  1:87776/1‑81 (MQ=255)                                                     cgAGCTGTTTAGCGCCGTACGGAAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTctct                   <  2:87742/81‑1 (MQ=255)                                                                 cgcCGTACGGAAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTc        >  1:87784/1‑80 (MQ=255)                                                                  gcCGTACGGAAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGa      <  2:87743/81‑1 (MQ=255)                                                                     gTACGGAAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATaa   >  1:87774/1‑81 (MQ=255)                                                                      tACGGAAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAAc  <  1:87744/81‑1 (MQ=255)                                                                           |                                                                            AGCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAGCTGTTTAGCGCCGTACGG‑AAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAACATCGCCCGCATTCTCTATTTGCAGGTCGATAAC  >  NC_000913/397483‑397631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑