Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,247,104	(A)7→8	coding (321/1116 nt)	malK →	maltose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,247,097	1	.	A	100.0%	118.3 / NA	33	A105E (GCA→GAA)	malK	maltose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (18/15);  total (18/15)

GTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGC‑AAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCATTTGCTGGATCGC  >  NC_000913/4247021‑4247170                                                                              |                                                                          gtgtTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGC‑aaa                                                                        <  2:848764/80‑1 (MQ=255)    tttCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGC‑aaaaaaa                                                                    <  1:848765/81‑1 (MQ=255)    tttCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGC‑aaaaaaa                                                                    <  2:848766/81‑1 (MQ=255)     ttCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGC‑aaaaa                                                                      >  1:848814/1‑78 (MQ=255)       cAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGC‑aaaaaaaa                                                                   >  2:848816/1‑78 (MQ=255)          tCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGt                                                               <  2:848767/81‑1 (MQ=255)             tACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGAt                                                            >  1:848811/1‑81 (MQ=255)              aCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTg                                                              <  1:848768/78‑1 (MQ=255)               cgcgCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGAtt                                                           >  2:848820/1‑80 (MQ=255)               cgcgCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGAtt                                                           <  2:848769/80‑1 (MQ=255)                  gcTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAAc                                                        >  1:848824/1‑80 (MQ=255)                      tATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCaa                                                     <  1:848770/79‑1 (MQ=255)                       aTCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAAcgc                                                  >  2:848838/1‑81 (MQ=255)                          cccACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGtt                                               <  2:848771/81‑1 (MQ=255)                          cccACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGtt                                               >  2:848827/1‑81 (MQ=255)                             aCCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAAc                                            <  1:848772/81‑1 (MQ=255)                                  tCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAg                                         <  1:848773/79‑1 (MQ=255)                                    aGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTgg                                     >  1:848830/1‑81 (MQ=255)                                    aGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTgg                                     >  2:848832/1‑81 (MQ=255)                                       aGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCg                                   >  1:848833/1‑80 (MQ=255)                                          aGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAg                               <  1:848774/81‑1 (MQ=255)                                          aGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAg                               >  2:848817/1‑81 (MQ=255)                                               aCATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTa                          >  2:848835/1‑81 (MQ=255)                                                cATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTAc                         <  2:848775/81‑1 (MQ=255)                                                   gTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAAc                      >  1:848826/1‑81 (MQ=255)                                                      aTTTGGCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTgg                   >  1:848844/1‑81 (MQ=255)                                                          ggCCTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCa               >  1:848848/1‑81 (MQ=255)                                                            ccTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCAtt             <  2:848776/81‑1 (MQ=255)                                                             cTGAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCAttt            <  2:848777/81‑1 (MQ=255)                                                               gAAACTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCATTTGc          >  1:848865/1‑81 (MQ=255)                                                                aaaCTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCATTTg           <  2:848778/79‑1 (MQ=255)                                                                 aaCTGGCTGGCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCATTTGCTg        >  2:848829/1‑81 (MQ=255)                                                                   ctggctggCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCATTTGCTGGa      <  2:848779/81‑1 (MQ=255)                                                                   ctggctggCGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCATTTGCTGGa      <  1:848780/81‑1 (MQ=255)                                                                     ggctggcGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCATTTGCTGGATc    >  2:848831/1‑81 (MQ=255)                                                                      gctggcGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCATTTGCTGGATc    <  2:848781/80‑1 (MQ=255)                                                                      gctggcGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCATTTGCTGGATCg   >  2:848862/1‑81 (MQ=255)                                                                        tggcGCAAAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCATTTGCTGGATCGc  >  1:848836/1‑80 (MQ=255)                                                                              |                                                                          GTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGC‑AAAAAAAGAGGTGATTAACCAACGCGTTAACCAGGTGGCGGAAGTGCTACAACTGGCGCATTTGCTGGATCGC  >  NC_000913/4247021‑4247170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑