Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,268,487	(T)7→8	intergenic (+180/+322)	tyrB → / ← yjbS	tyrosine aminotransferase/uncharacterized protein YjbS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,268,480	1	.	T	100.0%	20.5 / NA	7	intergenic (+173/+329)	tyrB/yjbS	tyrosine aminotransferase/uncharacterized protein YjbS
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (1/6);  total (1/6)

CGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAAT  >  NC_000913/4268401‑4268533                                                                                 |                                                      cGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑t                                                      >  2:853917/1‑81 (MQ=255)               gaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGa                                         <  1:853897/81‑1 (MQ=255)                  aataaACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGt                                       >  2:853918/1‑80 (MQ=255)                      aaCGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGtaataa                                  <  1:853898/81‑1 (MQ=255)                      aaCGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGtaata                                   <  2:853899/80‑1 (MQ=255)                                       tATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAAc                  <  1:853900/80‑1 (MQ=255)                                            ttAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCatta             <  2:853901/80‑1 (MQ=255)                                                       ccAATTATCTGGAATTCCTTATCCTGTTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTtaat  <  1:853902/80‑1 (MQ=255)                                                                                 |                                                      CGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTG‑TTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAAT  >  NC_000913/4268401‑4268533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑