Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,274,110	(T)7→8	intergenic (‑239/‑15)	uvrA ← / → ssb	excision nuclease subunit A/ssDNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,274,103	1	.	T	100.0%	122.0 / NA	34	intergenic (‑232/‑22)	uvrA/ssb	excision nuclease subunit A/ssDNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (26/8);  total (26/8)

GTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGA‑TTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGAC  >  NC_000913/4274023‑4274178                                                                                  |                                                                            gtttCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGa                                                                              >  1:855212/1‑81 (MQ=255)  tttCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGA‑t                                                                            >  2:855217/1‑81 (MQ=255)        ggAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGA‑ttttttt                                                                      >  2:855209/1‑81 (MQ=255)        ggAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGA‑ttttttt                                                                      >  2:855226/1‑81 (MQ=255)         gAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGA‑tttttttt                                                                     >  2:855210/1‑80 (MQ=255)                  tCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGaca                                                             >  2:855199/1‑81 (MQ=255)                   cacaACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGacac                                                            >  2:855197/1‑81 (MQ=255)                   cacaACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGacac                                                            >  1:855207/1‑81 (MQ=255)                    acaacaTAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGacac                                                            >  1:855221/1‑80 (MQ=255)                         aTAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACAt                                                      <  2:855169/81‑1 (MQ=255)                            gTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGc                                                   <  1:855170/81‑1 (MQ=255)                             tAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGcc                                                  <  1:855171/81‑1 (MQ=255)                                aaGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCcag                                                >  1:855201/1‑80 (MQ=255)                                    gcTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCagag                                           >  1:855219/1‑81 (MQ=255)                                    gcTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCagag                                           >  2:855240/1‑81 (MQ=255)                                       aTTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCg                                        >  2:855239/1‑81 (MQ=255)                                        ttGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGt                                       <  1:855172/81‑1 (MQ=255)                                           gTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTaaa                                    >  1:855214/1‑81 (MQ=255)                                            tAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTaaa                                    >  2:855215/1‑80 (MQ=255)                                             aaTGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAAc                                   >  1:855220/1‑80 (MQ=255)                                                ggTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAgg                               >  1:855243/1‑81 (MQ=255)                                                  tACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGtt                             <  2:855173/81‑1 (MQ=255)                                                     aaTCGCGCGTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTAtt                          >  1:855225/1‑81 (MQ=255)                                                        cgcgcgTTTACACTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATtctc                       >  1:855204/1‑81 (MQ=255)                                                                 acacTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAAt              >  2:855232/1‑81 (MQ=255)                                                                  cacTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAAt              <  1:855174/80‑1 (MQ=255)                                                                  cacTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATc             >  2:855235/1‑81 (MQ=255)                                                                  cacTTATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATc             >  2:855213/1‑81 (MQ=255)                                                                      tATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTggg         >  2:855267/1‑81 (MQ=255)                                                                      tATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTggg         >  2:855250/1‑81 (MQ=255)                                                                      tATTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTggg         >  2:855208/1‑81 (MQ=255)                                                                       aTTCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGt        >  2:855211/1‑81 (MQ=255)                                                                        ttCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTc       <  2:855175/81‑1 (MQ=255)                                                                         tCAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGt        >  1:855251/1‑79 (MQ=255)                                                                          cAGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAg     >  1:855258/1‑81 (MQ=255)                                                                           aGAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAg     >  1:855252/1‑80 (MQ=255)                                                                            gAACGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGa   <  2:855176/81‑1 (MQ=255)                                                                             aaCGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGAc  >  1:855224/1‑81 (MQ=255)                                                                             aaCGATTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGAc  >  2:855236/1‑81 (MQ=255)                                                                                  |                                                                            GTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGA‑TTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGAC  >  NC_000913/4274023‑4274178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑