Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,506,193	(A)8→9	coding (907/1119 nt)	yjhC →	KpLE2 phage‑like element; putative oxidoreductase YjhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,506,185	1	.	A	100.0%	78.1 / NA	22	G300E (GGA→GAA)	yjhC	KpLE2 phage‑like element; putative oxidoreductase YjhC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (11/11);  total (11/11)

CACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑AAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTG  >  NC_000913/4506107‑4506259                                                                                |                                                                           cacaAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑aa                                                                          <  1:910717/81‑1 (MQ=255)  acaAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑aaa                                                                         <  2:910718/81‑1 (MQ=255)     agaagaagaTGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑aaaaaa                                                                      <  1:910719/81‑1 (MQ=255)        agaagaTGATGATCGTCGGAAAGGCAATATGACCTCAGATATGGATGGCGCTATAGCATATGGTCATCCAGG‑aaaaaaaaa                                                                   >  1:910770/1‑80 (MQ=255)         gaagaTGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑aaaaaaaaaa                                                                  >  1:910758/1‑79 (MQ=255)          aagaTGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑aaaaaaaaaca                                                                 <  1:910720/81‑4 (MQ=255)               gatgatCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCAt                                                              >  2:910776/1‑80 (MQ=255)                 tgatCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTAt                                                           <  2:910721/81‑1 (MQ=255)                   atCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATgg                                                         <  1:910722/81‑1 (MQ=255)                         ggAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTgtc                                                   <  1:910723/81‑3 (MQ=255)                         ggAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTgt                                                    <  1:910724/80‑2 (MQ=255)                              ggCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGttt                                              <  2:910725/81‑1 (MQ=255)                                 aaTATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTtaa                                            >  1:910768/1‑80 (MQ=255)                                               aaTGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGt                             >  1:910771/1‑81 (MQ=255)                                                     tggCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATtcc                       >  1:910778/1‑81 (MQ=255)                                                       gcgcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATtcctc                     >  2:910787/1‑81 (MQ=255)                                                       gcgcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATtcct                      <  2:910726/80‑1 (MQ=255)                                                        cgcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATtcctcc                    <  1:910727/81‑1 (MQ=255)                                                         gcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCa                   >  1:910767/1‑81 (MQ=255)                                                         gcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCa                   >  2:910777/1‑81 (MQ=255)                                                          cTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCat                  <  1:910728/81‑1 (MQ=255)                                                                cATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATc            <  1:910729/81‑1 (MQ=255)                                                                    tGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCctc         <  2:910730/80‑1 (MQ=255)                                                                      gTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCtgt      >  2:910773/1‑81 (MQ=255)                                                                         aTCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTgtggt   >  1:910779/1‑81 (MQ=255)                                                                         aTCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTgtggt   >  2:910782/1‑81 (MQ=255)                                                                         aTCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTgtgg    <  1:910731/80‑1 (MQ=255)                                                                          tCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTgtggtg  >  1:910732/1‑81 (MQ=255)                                                                                |                                                                           CACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑AAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTG  >  NC_000913/4506107‑4506259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑