| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 561,972 | (A)7→8 | coding (2276/2604 nt) | sfmD → | putative fimbrial usher protein SfmD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 561,965 | 1 | . | A | 96.2% | 90.2 / ‑3.9 | 26 | coding (2269/2604 nt) | sfmD | putative fimbrial usher protein SfmD |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (16/9); total (16/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTG‑AAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGC > NC_000913/561888‑562040 | tGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTG‑aaa < 2:122387/81‑1 (MQ=255) gATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTG‑aaa > 2:122422/1‑80 (MQ=255) tGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTG‑aaaaaaaa > 2:122438/1‑79 (MQ=255) aTGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTG‑aaaaaaaatat > 2:122423/1‑76 (MQ=255) gCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAAATATTag > 1:122429/1‑81 (MQ=255) gCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTG‑AAAAAAaaatatt < 2:122388/80‑4 (MQ=255) aCGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAAATATTagcag > 2:122426/1‑81 (MQ=255) gTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGt < 2:122389/81‑1 (MQ=255) tATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTtgt > 2:122431/1‑81 (MQ=255) aTCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTtgtg > 1:122441/1‑81 (MQ=255) ggTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCg < 1:122390/81‑1 (MQ=255) tCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGcgcg > 1:122430/1‑81 (MQ=255) gcTTGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTgg > 2:122433/1‑81 (MQ=255) gATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTc < 1:122391/80‑1 (MQ=255) gATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCg > 2:122439/1‑81 (MQ=255) aTACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGt > 1:122435/1‑81 (MQ=255) aCCAATACGATGGGGAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGc > 1:122447/1‑81 (MQ=255) gATGGGGAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTg < 1:122392/81‑1 (MQ=255) ggggAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATa > 2:122442/1‑81 (MQ=255) gggAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATAc > 1:122462/1‑81 (MQ=255) gggAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATAc > 1:122437/1‑81 (MQ=255) ggAATTCCATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATAcc > 2:122440/1‑81 (MQ=255) aaTTCCATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCg > 2:122432/1‑81 (MQ=255) cATCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAg > 1:122445/1‑81 (MQ=255) aTCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAgg > 1:122458/1‑81 (MQ=255) aTCGATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAgg < 1:122393/81‑1 (MQ=255) gATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCg < 2:122394/80‑1 (MQ=255) gATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCg < 1:122396/80‑1 (MQ=255) gATGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCg < 1:122395/80‑1 (MQ=255) tGTTGAAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGc < 2:122397/81‑1 (MQ=255) | TGATGCTGTATGCCACGGTTTATCGGTATAACCGTATCGCGCTTGATACCAATACGATGGGGAATTCCATCGATGTTG‑AAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGC > NC_000913/561888‑562040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |