Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	638,568	(T)6→7	coding (6/747 nt)	dsbG ←	protein sulfenic acid reductase DsbG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	638,562	1	.	T	100.0%	60.9 / NA	18	K4K (AAG→AAA)	dsbG	protein sulfenic acid reductase DsbG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (10/8);  total (10/8)

GCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATC‑TTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGG  >  NC_000913/638497‑638636                                                                   |                                                                           gCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTa                                                               <  2:136740/80‑1 (MQ=255)   ttttACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTAAtt                                                            >  1:136762/1‑81 (MQ=255)           gAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCt                                                      <  2:136741/79‑1 (MQ=255)                  aaGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCTTTtcagt                                               >  2:136763/1‑79 (MQ=255)                        ctctGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCa                                        <  1:136742/80‑1 (MQ=255)                         tctGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCaa                                       >  2:136770/1‑80 (MQ=255)                             cGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTc                                  <  1:136743/81‑1 (MQ=255)                              gAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTc                                  >  1:136776/1‑80 (MQ=255)                                 ggagATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGt                              <  2:136744/78‑1 (MQ=255)                                    gATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTaa                            >  1:136768/1‑80 (MQ=255)                                     aTTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTaaaa                          <  2:136745/81‑1 (MQ=255)                                       tGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAgg                        >  1:136767/1‑81 (MQ=255)                                         cAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCa                      >  2:136766/1‑81 (MQ=255)                                             aaGCAGAGCCAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAAc                  <  1:136746/81‑1 (MQ=255)                                                      cAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTg         <  2:136747/81‑1 (MQ=255)                                                      cAGTAAAAGTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTg         >  2:136758/1‑81 (MQ=255)                                                            aaGTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAg   >  1:136773/1‑81 (MQ=255)                                                              gTATCTTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAgg  >  2:136779/1‑80 (MQ=255)                                                                   |                                                                           GCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATC‑TTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGG  >  NC_000913/638497‑638636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑