Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,903,7840GT23.3% 63.2 / 15.8 30A235S (GCA→TCA) manYmannose‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base G (9/14);  new base T (3/4);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TCTTCTACCTCGGCTTCGTAACCGCAGCATTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGGCAGTGCTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCTGCTCAGGCAGCTGGTAACAACGATCTC  >  NC_000913/1903707‑1903864
                                                                             |                                                                                
tcttctACCTCGGCTTCGTAACCGCAGCATTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGGCAg                                                                               >  1:335604/1‑81 (MQ=255)
     tACCTCGGCTTCGTAACCGCAGTATTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGGCAGTGttc                                                                          >  2:335594/1‑81 (MQ=255)
     tACCTCGGCTTCGTAACCGCAGCATTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGGCAGTGttc                                                                          >  1:335595/1‑81 (MQ=255)
      aCCTCGGCTTCGTAACCGCAGCATTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGGGAGTGttc                                                                          >  1:335610/1‑80 (MQ=255)
       ccTCGGCTTCGTAACCGCAGCATTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGGCAGTgtt                                                                           <  2:335562/78‑3 (MQ=255)
       ccTCGGCTTCGTAACCGCAGCATTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGGCAGTGttct                                                                         <  2:335561/80‑1 (MQ=255)
       ccTCGGCTTCGTAACCGCAGCATTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGGCAGTGttc                                                                          <  1:335560/79‑1 (MQ=255)
           ggCTTCGTAACCGCAGCATTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGTCAGTGTTCTACAt                                                                     <  2:335563/80‑1 (MQ=255)
                cGTAACCGCAGCATTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGTCAGTGTTCTACATCCAACt                                                               >  2:335601/1‑81 (MQ=255)
                 gTAACCGCAGCATTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGTCAGTGTTCTACATCCAACtt                                                              <  2:335564/81‑1 (MQ=255)
                        cagcaTTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCg                                                        >  2:335597/1‑80 (MQ=255)
                         agcaTTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGTCAGTGTTCTACATCCAACTTAGCCCGa                                                       >  2:335613/1‑80 (MQ=255)
                               cACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACa                                                 <  1:335565/80‑1 (MQ=255)
                                aCCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGTCAGTGTTCTACATCCAACTTAGCCCGAAATACaa                                                <  1:335567/80‑1 (MQ=255)
                                aCCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAAc                                               <  1:335566/81‑1 (MQ=255)
                                 ccAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGTCAGTGTTCTACATCCAACTTAGCCCGAAATACAAcc                                              >  2:335596/1‑81 (MQ=255)
                                         aaCCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGTCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGc                                       <  2:335568/80‑1 (MQ=255)
                                               gTTGCTCTGGGTGTGATTGGTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTgc                                 <  2:335569/80‑1 (MQ=255)
                                               gTTGCTCTGGGTGTGATTGGTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTgc                                 <  1:335570/80‑1 (MQ=255)
                                                  gCTCTGGGTGTGATTGGTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCt                             >  1:335599/1‑81 (MQ=255)
                                                  gCTCTGGGTGTGATTGGTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCt                             >  2:335615/1‑81 (MQ=255)
                                                     ctGGGTGTGATTGGTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCTGCt                          >  1:335600/1‑81 (MQ=255)
                                                        ggTGTGATTGGTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCTGCt                          <  2:335572/78‑1 (MQ=255)
                                                        ggTGTGATTGGTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCTGCt                          <  2:335571/78‑1 (MQ=255)
                                                        ggTGTGATTGGTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCTGCTCAg                       >  2:335607/1‑81 (MQ=255)
                                                            tgATTGGTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCTGCTCAGGCAg                   <  2:335574/81‑1 (MQ=255)
                                                            tgATTGGTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCTGCTCAGGCAg                   <  1:335573/81‑1 (MQ=255)
                                                                  gTACTGTTATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCTGCTCAGGCAGCTGGt              <  1:335575/80‑1 (MQ=255)
                                                                        ttATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCGTGCTCAGGCAGCTGGTaacaac        <  2:335577/80‑1 (MQ=255)
                                                                        ttATGGCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCTGCTCAGGCAGCTGGTAACAACg       <  2:335576/81‑1 (MQ=255)
                                                                             gCAGTGTTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCTGCTCAGGCAGCTGGTAACAACGAtctc  <  1:335578/81‑1 (MQ=255)
                                                                             |                                                                                
TCTTCTACCTCGGCTTCGTAACCGCAGCATTCACCAACTTTAACCTGGTTGCTCTGGGTGTGATTGGTACTGTTATGGCAGTGCTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCTGCTCAGGCAGCTGGTAACAACGATCTC  >  NC_000913/1903707‑1903864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: