Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	37,137	A→C	C230G (TGT→GGT)	caiC ←	carnitine‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	37,137	0	A	C	100.0%	168.4 / NA	47	C230G (TGT→GGT)	caiC	carnitine‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/30);  total (17/30)

AGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAG  >  NC_000913/37062‑37213                                                                            |                                                                             aGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGc                                                                         <  1:8416/81‑1 (MQ=255) aGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGc                                                                         <  2:8415/81‑1 (MQ=255)  gAAGGCGCGGGCGCTGTATTTCTCGACTAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCCGGc                                                                         >  1:8457/1‑80 (MQ=255)  gAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCa                                                                        <  2:8417/81‑1 (MQ=255)  gAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTAGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCa                                                                        <  2:8418/81‑1 (MQ=255)   aaGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCAGGAAGAAAACGCCGCCATCGCCGCAGTAACCTGGCaa                                                                       >  1:8467/1‑81 (MQ=255)    aGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAAt                                                                      >  1:8473/1‑81 (MQ=255)       cgcgGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGa                                                                   <  1:8419/81‑1 (MQ=255)          gggCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGatat                                                                >  1:8470/1‑81 (MQ=255)              gcTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGaaa                                                            >  2:8477/1‑81 (MQ=255)                  tATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACg                                                          <  1:8421/79‑1 (MQ=255)                  tATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGc                                                         <  2:8420/80‑1 (MQ=255)                       ctcGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCa                                                    <  2:8422/80‑1 (MQ=255)                            ccAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCg                                              <  1:8423/81‑1 (MQ=255)                            ccAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCg                                              >  2:8474/1‑81 (MQ=255)                                cacaAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAg                                          >  2:8488/1‑81 (MQ=255)                                cacaAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAg                                          <  1:8424/81‑1 (MQ=255)                                 acaAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAgg                                         >  2:8500/1‑81 (MQ=255)                                 acaAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAgg                                         <  2:8425/81‑1 (MQ=255)                                  caAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAgg                                         >  1:8482/1‑80 (MQ=255)                                   aaaGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAgg                                         >  1:8487/1‑79 (MQ=255)                                    aaGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAg                                      <  2:8426/81‑1 (MQ=255)                                       gTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACg                                   >  2:8486/1‑81 (MQ=255)                                        tGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGt                                  <  2:8427/81‑1 (MQ=255)                                           ccccGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCAt                               <  2:8429/81‑1 (MQ=255)                                           ccccGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCAt                               <  2:8428/81‑1 (MQ=255)                                              cGGCAGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGt                            <  1:8430/81‑1 (MQ=255)                                                  aGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACg                        >  2:8483/1‑81 (MQ=255)                                                  aGAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACg                        <  2:8431/81‑1 (MQ=255)                                                   gAAAACGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGc                       <  2:8432/81‑1 (MQ=255)                                                    aaaaCGCCGCCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCa                      <  2:8433/81‑1 (MQ=255)                                                        cgccgcCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTg                   >  2:8478/1‑80 (MQ=255)                                                        cgccgcCATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGc                  <  2:8434/81‑1 (MQ=255)                                                           cgccATCGCCGCAGTACCCTAGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGcaca               >  2:8490/1‑81 (MQ=255)                                                            gccATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCacac              <  1:8436/81‑1 (MQ=255)                                                            gccATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCacac              <  2:8437/81‑1 (MQ=255)                                                            gccATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCacac              <  2:8435/81‑1 (MQ=255)                                                            gccATCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCacac              <  1:8438/81‑1 (MQ=255)                                                               aTCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGc           >  1:8492/1‑81 (MQ=255)                                                               aTCGCCGCAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGc           >  1:8481/1‑81 (MQ=255)                                                                  gccgcAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCa         <  1:8440/80‑1 (MQ=255)                                                                  gccgcAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAg        <  1:8439/81‑1 (MQ=255)                                                                   ccgcAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAgg       <  2:8441/81‑1 (MQ=255)                                                                     gcAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCg     >  2:8489/1‑81 (MQ=255)                                                                     gcAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCg     <  1:8442/81‑1 (MQ=255)                                                                      cAGTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCgg    <  2:8443/81‑1 (MQ=255)                                                                        gTACCCTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAg  <  1:8444/81‑1 (MQ=255)                                                                            |                                                                             AGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAG  >  NC_000913/37062‑37213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑