Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	143,125	G→A	R116H (CGC→CAC)	yadG →	putative ABC transporter ATP‑binding protein YadG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	143,125	0	G	A	100.0%	93.5 / NA	26	R116H (CGC→CAC)	yadG	putative ABC transporter ATP‑binding protein YadG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/12);  total (14/12)

CCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGA  >  NC_000913/143048‑143202                                                                              |                                                                              cccGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAg                                                                            >  1:33617/1‑81 (MQ=255)  ccGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGc                                                                           <  1:33577/81‑1 (MQ=255)      ttGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGaa                                                                        >  2:33615/1‑80 (MQ=255)       tGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGat                                                                        >  2:33608/1‑78 (MQ=255)       tGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGaaa                                                                       <  1:33578/80‑1 (MQ=255)           aCCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTAt                                                                  >  1:33628/1‑81 (MQ=255)              gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCtt                                                               <  2:33579/81‑1 (MQ=255)                        ttGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCTTAAACAACTCg                                                     <  1:33580/81‑1 (MQ=255)                           tggtgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGAt                                                   <  2:33581/80‑1 (MQ=255)                           tggtgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGAt                                                   <  2:33582/80‑1 (MQ=255)                             gtgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCt                                                 <  2:33583/80‑1 (MQ=255)                             gtgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCTa                                                >  1:33623/1‑81 (MQ=255)                              tgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCTAt                                               >  2:33621/1‑81 (MQ=255)                              tgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCTAt                                               <  1:33584/81‑1 (MQ=255)                                   caggcaggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCTATgggg                                           >  2:33619/1‑80 (MQ=255)                                     ggcaggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGaaa                                        <  2:33585/81‑1 (MQ=255)                                      gcaggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGaaaa                                       <  2:33586/81‑1 (MQ=255)                                            tactacGGCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaac                                 <  1:33587/81‑1 (MQ=255)                                                  ggCGTGGAGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt                           >  1:33614/1‑81 (MQ=255)                                                         aGCGCAAAGAAGCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTa                    >  2:33618/1‑81 (MQ=255)                                                                  aagCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTg           >  1:33639/1‑81 (MQ=255)                                                                  aagCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTg           >  1:33627/1‑81 (MQ=255)                                                                  aagCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTg           >  1:33624/1‑81 (MQ=255)                                                                   agCGTACATCCACAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTgg          >  2:33648/1‑81 (MQ=255)                                                                        aCATCCACAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGa     >  1:33636/1‑81 (MQ=255)                                                                           tCCACAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGa  <  1:33588/81‑1 (MQ=255)                                                                              |                                                                              CCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGA  >  NC_000913/143048‑143202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑