Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	922,079	T→G	L578R (CTG→CGG)	macB →	ABC‑type tripartite efflux pump ATP binding/membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	922,079	0	T	G	100.0%	84.3 / NA	24	L578R (CTG→CGG)	macB	ABC‑type tripartite efflux pump ATP binding/membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/17);  total (7/17)

AATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCTTACCC  >  NC_000913/922008‑922155                                                                        |                                                                             aaTTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCt                                                                     <  1:190632/81‑1 (MQ=255) aaTTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCt                                                                     <  1:190631/81‑1 (MQ=255)      gCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTc                                                                 <  1:190634/80‑1 (MQ=255)      gCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCg                                                                <  1:190633/81‑1 (MQ=255)         ttCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTg                                                             <  1:190635/81‑1 (MQ=255)            gCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGgc                                                           <  2:190636/80‑1 (MQ=255)             cATGGCTGTATGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGgcgc                                                         >  1:190671/1‑81 (MQ=255)              aTGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGctcg                                                        >  2:190666/1‑81 (MQ=255)                  cTGTAGGTGCGCGAGCAAGCGATGTTTTGCAGCAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTgg                                                    <  2:190637/81‑1 (MQ=255)                   tGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTgg                                                    >  1:190667/1‑80 (MQ=255)                         tGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAac                                             <  1:190638/81‑1 (MQ=255)                              gAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgt                                        >  2:190670/1‑81 (MQ=255)                               aGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgtc                                       <  1:190640/81‑1 (MQ=255)                               aGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgtc                                       <  1:190639/81‑1 (MQ=255)                                 cAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgtcac                                     <  2:190641/81‑1 (MQ=255)                                  aaGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgtcact                                    <  1:190642/81‑1 (MQ=255)                                   aGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgtcactg                                   >  2:190672/1‑81 (MQ=255)                                               cAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCttt                        <  1:190643/80‑1 (MQ=255)                                                          gATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCt            >  2:190674/1‑81 (MQ=255)                                                           aTCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCtt           <  2:190644/81‑1 (MQ=255)                                                            tCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCttt          <  2:190645/81‑1 (MQ=255)                                                                aGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCtt      <  1:190646/81‑1 (MQ=255)                                                                    gTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCTTAccc  >  1:190662/1‑81 (MQ=255)                                                                    gTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCTTAccc  <  1:190647/81‑1 (MQ=255)                                                                        |                                                                             AATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCTTACCC  >  NC_000913/922008‑922155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑