Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	925,507	T→A	S748R (AGT→AGA)	clpA →	ATP‑dependent Clp protease ATP‑binding subunit ClpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	925,507	0	T	A	100.0%	70.5 / NA	20	S748R (AGT→AGA)	clpA	ATP‑dependent Clp protease ATP‑binding subunit ClpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (10/10);  total (10/10)

GGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAG  >  NC_000913/925438‑925584                                                                      |                                                                              ggtggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGca                                                                    >  2:191282/1‑81 (MQ=255)     gACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAg                                                                <  2:191252/81‑1 (MQ=255)        ggcggTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCg                                                             <  1:191253/81‑1 (MQ=255)           ggtcaggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCgg                                                            >  2:191294/1‑79 (MQ=255)               aggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAagca                                                       <  2:191254/80‑1 (MQ=255)                 gtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAgcg                                                    >  2:191289/1‑81 (MQ=255)                    aCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCAt                                                 <  1:191255/81‑1 (MQ=255)                     ccGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCAtt                                                <  1:191256/81‑1 (MQ=255)                     ccGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCAt                                                 >  1:191290/1‑80 (MQ=255)                         cgcgCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATc                                            >  2:191292/1‑81 (MQ=255)                            gcTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGa                                         <  2:191257/81‑1 (MQ=255)                             cTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGAt                                        >  1:191295/1‑81 (MQ=255)                                  tAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGt                                     >  1:191297/1‑79 (MQ=255)                                   aaaGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTtag                                  >  2:191286/1‑81 (MQ=255)                                   aaaGAGAAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTtag                                  >  2:191296/1‑81 (MQ=255)                                       agaAAAATGAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTtaggtag                              <  2:191258/81‑1 (MQ=255)                                                aGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTc                       >  2:191299/1‑79 (MQ=255)                                                aGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCa                      <  2:191260/80‑1 (MQ=255)                                                aGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCa                      <  1:191259/80‑1 (MQ=255)                                                     aCTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTcc                 <  1:191261/80‑1 (MQ=255)                                                                   gagaGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAagag  <  2:191262/81‑1 (MQ=255)                                                                      |                                                                              GGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAG  >  NC_000913/925438‑925584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑