Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	932,104	A→C	intergenic (‑54/‑491)	trxB ← / → lrp	thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	932,104	0	A	C	100.0%	69.7 / NA	20	intergenic (‑54/‑491)	trxB/lrp	thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (8/12);  total (8/12)

GTTTACTGTGTTTGGTCGTGCCCATGAGATCCCCATAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGACGTAAAAAAAGAGTATGACGATTTTGTTAACAATTTGTGCAATCGGCAGCATCGATAAGCAGGTCAAA  >  NC_000913/932026‑932172                                                                               |                                                                     gTTTACTGTGTTTGGTCGTGCCCATGAGATCCCCATAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCg                                                                    <  1:192454/81‑1 (MQ=255)             tGGTCGTGCCCATGAGATCCCCATAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAagag                                                         <  1:192455/80‑1 (MQ=255)              ggTCGTGCCCATGAGATCCCCATAGTTGTTGGCAGACAATGGGGAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTa                                                       >  2:192486/1‑81 (MQ=255)                tCGTGCCCATGAGATCCCCATAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATg                                                     <  2:192456/81‑1 (MQ=255)                  gTGCCCATGAGATCCCCATAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGa                                                    <  2:192457/80‑1 (MQ=255)                   tGCCCATGAGATCCCCATAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACg                                                  <  1:192458/81‑1 (MQ=255)                        aTGAGATCCCCATAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACGAtttt                                             >  2:192495/1‑81 (MQ=255)                        aTGAGATCCCCATAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACGAtttt                                             <  1:192459/81‑1 (MQ=255)                           agaTCCCCATAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACGATTTTGtt                                          <  2:192461/81‑1 (MQ=255)                           agaTCCCCATAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACGATTTTGt                                           >  2:192483/1‑80 (MQ=255)                           agaTCCCCATAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACGATTTTGt                                           <  1:192460/80‑1 (MQ=255)                                cccATAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACGATTTTGTTAACa                                      <  2:192462/80‑1 (MQ=255)                                   aTAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACGATTTTGTTAACAAttt                                  <  1:192463/81‑1 (MQ=255)                                    tAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACGATTTTGTTAACAATTtg                                 >  2:192491/1‑81 (MQ=255)                                      gttgttGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACGATTTTGTTAACAATTtgtg                               <  1:192464/81‑1 (MQ=255)                                       ttgttgGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACGATTTTGTTAACAATTtgtg                               >  2:192493/1‑80 (MQ=255)                                        tgttgGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACGATTTTGTTAACAATTTGTGc                              >  1:192492/1‑80 (MQ=255)                                           tgGCAGACAATGGGCAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACGATTTTGTTAACAATTTGTGCAATc                          >  1:192494/1‑81 (MQ=255)                                                         cAGGATTGTAGGGAATTTACAGCCGTAAAAAAAGAGTATGACGATTTTGTTAACAATTTGTGCAATCGGCAGCATCGATaa            <  1:192465/81‑1 (MQ=255)                                                                   gggAATTTACAGCCGTAAAAAAAGAGTATGACGATTTTGTTAACAATTTGTGCAATCGGCAGCATCGATAAGCAGGTCaaa  >  1:192564/1‑81 (MQ=255)                                                                               |                                                                     GTTTACTGTGTTTGGTCGTGCCCATGAGATCCCCATAGTTGTTGGCAGACAATGGGCAGGATTGTAGGGAATTTACAGACGTAAAAAAAGAGTATGACGATTTTGTTAACAATTTGTGCAATCGGCAGCATCGATAAGCAGGTCAAA  >  NC_000913/932026‑932172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑