Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	974,815	A→G	E166G (GAA→GGA)	mukF →	chromosome partitioning protein MukF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	974,815	0	A	G	100.0%	123.8 / NA	35	E166G (GAA→GGA)	mukF	chromosome partitioning protein MukF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/15);  total (20/15)

CCGCCGAAGAGGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGAAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAG  >  NC_000913/974743‑974891                                                                         |                                                                             ccgccgAAGAGGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTc                                                                       <  2:199965/80‑1 (MQ=255) ccgccgAAGAGGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTc                                                                       >  1:200009/1‑80 (MQ=255)      gAAGAGGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGt                                                                 <  1:199966/81‑1 (MQ=255)        agagGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTAt                                                               <  2:199967/81‑1 (MQ=255)        agagGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTAt                                                               >  1:200015/1‑81 (MQ=255)         gagGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATc                                                              <  1:199968/81‑1 (MQ=255)         gagGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATc                                                              >  1:200017/1‑81 (MQ=255)         gagGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATc                                                              >  1:200012/1‑81 (MQ=255)            ggCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGAc                                                           <  2:199969/81‑1 (MQ=255)              cGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCt                                                         >  2:200010/1‑81 (MQ=255)                gTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGa                                                       <  1:199970/81‑1 (MQ=255)                 tgatgaATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGAc                                                      <  2:199971/81‑1 (MQ=255)                        tttCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGt                                               >  2:200027/1‑81 (MQ=255)                         ttCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTc                                              >  2:200029/1‑81 (MQ=255)                          tCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCt                                             >  1:200004/1‑81 (MQ=255)                            aCTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTg                                            <  1:199972/80‑1 (MQ=255)                               ggCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATg                                         >  2:200019/1‑80 (MQ=255)                                  aCCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGAc                                      >  1:200020/1‑80 (MQ=255)                                  aCCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACg                                     <  1:199973/81‑1 (MQ=255)                                  aCCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACg                                     <  2:199974/81‑1 (MQ=255)                                     gTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGaa                                   >  2:200018/1‑80 (MQ=255)                                     gTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGaa                                   >  1:200008/1‑80 (MQ=255)                                          gTCTATGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAAcagcag                             >  1:200013/1‑81 (MQ=255)                                               tGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAAcagcagcagca                        <  2:199975/81‑1 (MQ=255)                                               tGCGCCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAAcagcagcag                          <  1:199976/79‑1 (MQ=255)                                                 cgcCACTGAAATATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAgg                      >  2:200005/1‑81 (MQ=255)                                                         aaaTATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGa               <  1:199977/80‑1 (MQ=255)                                                          aaTATTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACg             <  2:199978/81‑1 (MQ=255)                                                             atTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGata          >  2:200014/1‑81 (MQ=255)                                                             atTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGata          >  1:200024/1‑81 (MQ=255)                                                             atTCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGata          >  1:200011/1‑81 (MQ=255)                                                              ttCGGTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGatat         >  1:200031/1‑81 (MQ=255)                                                                  gTAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGcc     <  1:199979/81‑1 (MQ=255)                                                                   tAGCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGcc     >  1:200026/1‑80 (MQ=255)                                                                     gCAGGAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAg  >  1:200023/1‑81 (MQ=255)                                                                         |                                                                             CCGCCGAAGAGGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTGAAATATTCGGTAGCAGAAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAG  >  NC_000913/974743‑974891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑