Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	979,598	C→T	N1091N (AAC→AAT)	mukB →	chromosome partitioning protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	979,598	0	C	T	100.0%	143.2 / NA	40	N1091N (AAC→AAT)	mukB	chromosome partitioning protein MukB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/21);  total (19/21)

CGCAACTGAGCAATAACCGTTCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAACCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGACCGCCAAAGCGGGCT  >  NC_000913/979522‑979674                                                                             |                                                                             cgcAACTGAGCAATAACCGTTCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTg                                                                           <  2:201020/80‑1 (MQ=255)  gcAACTGAGCAATAACCGTTCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGAc                                                                         <  2:201021/81‑1 (MQ=255)    aaCTGAGCAATAACCGTTCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGAccc                                                                       <  2:201022/81‑1 (MQ=255)        gAGCAATAACCGTTCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCa                                                                    >  2:201073/1‑80 (MQ=255)          gCAATAACCGTTCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAAc                                                                 <  1:201023/81‑1 (MQ=255)           cAATAACCGTTCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACt                                                                <  2:201025/81‑1 (MQ=255)           cAATAACCGTTCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACt                                                                <  2:201024/81‑1 (MQ=255)            aaTAACCGTTCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTg                                                               >  1:201075/1‑81 (MQ=255)             aTAACCGTTCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTgc                                                              <  1:201026/81‑1 (MQ=255)              tAACCGTTCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTgcg                                                             <  2:201027/81‑1 (MQ=255)                 ccGTTCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCaa                                                          <  1:201028/81‑1 (MQ=255)                    ttCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCt                                                       <  1:201029/81‑1 (MQ=255)                     tCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTg                                                      >  1:201077/1‑81 (MQ=255)                        cgccgcAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAg                                                   <  2:201030/81‑1 (MQ=255)                         gccgcAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGc                                                  <  2:201031/81‑1 (MQ=255)                            gcAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCgg                                                >  1:201084/1‑80 (MQ=255)                              aaTCAACTGGAAAAAGCGCTTAGCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGAt                                             >  2:201093/1‑81 (MQ=255)                                  aaCTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACt                                         >  1:201074/1‑81 (MQ=255)                                      ggAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACttt                                       >  1:201078/1‑79 (MQ=255)                                       gAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTg                                      <  2:201032/79‑1 (MQ=255)                                           aaGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATgc                                >  1:201080/1‑81 (MQ=255)                                           aaGCGCTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATgc                                <  2:201033/81‑1 (MQ=255)                                             gcgcTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATgcgc                              >  1:201087/1‑81 (MQ=255)                                              cgcTTACCTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGcgcg                             <  1:201034/81‑1 (MQ=255)                                                     cTTCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGt                      <  1:201035/81‑1 (MQ=255)                                                      ttCTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAgg                       >  2:201072/1‑79 (MQ=255)                                                        cTGCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGt                   >  2:201082/1‑81 (MQ=255)                                                          gCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGa                 >  2:201091/1‑81 (MQ=255)                                                          gCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGa                 >  2:201083/1‑81 (MQ=255)                                                          gCGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGa                 >  2:201064/1‑81 (MQ=255)                                                           cGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTg                  <  2:201037/79‑1 (MQ=255)                                                           cGAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGAc                <  1:201036/81‑1 (MQ=255)                                                            gAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGAcc               >  1:201108/1‑81 (MQ=255)                                                            gAAGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGAcc               >  1:201100/1‑81 (MQ=255)                                                             aaGCGGAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGACCg              >  2:201076/1‑81 (MQ=255)                                                                 ggAGATGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGACCGCCaa          <  2:201038/81‑1 (MQ=255)                                                                   agaTGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGACCGCCAAAg        >  2:201079/1‑81 (MQ=255)                                                                   agaTGGACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGACCGCCAAAg        <  1:201039/81‑1 (MQ=255)                                                                        gACAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGACCGCCAAAGCGGGc   <  1:201040/81‑1 (MQ=255)                                                                         aCAATCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGACCGCCAAAGCGGGCt  >  2:201092/1‑81 (MQ=255)                                                                             |                                                                             CGCAACTGAGCAATAACCGTTCACGCCGCAATCAACTGGAAAAAGCGCTTACCTTCTGCGAAGCGGAGATGGACAACCTGACCCGCAAACTGCGCAAGCTGGAGCGGGATTACTTTGAGATGCGCGAGCAGGTAGTGACCGCCAAAGCGGGCT  >  NC_000913/979522‑979674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑