Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	982,175	A→G	I377V (ATC→GTC)	ldtD →	L,D‑transpeptidase LdtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	982,175	0	A	G	100.0%	64.0 / NA	19	I377V (ATC→GTC)	ldtD	L,D‑transpeptidase LdtD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/10);  total (9/10)

GCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCAT  >  NC_000913/982099‑982248                                                                             |                                                                          gcccgcccAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGGTCAt                                                                       >  2:201568/1‑81 (MQ=255)     gcccAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGGTCATGGt                                                                    >  2:201557/1‑80 (MQ=255)          gCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGGTCATGGTTAACAt                                                              >  1:201559/1‑81 (MQ=255)            gTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGGTCATGGTTAACATTc                                                            <  1:201531/81‑1 (MQ=255)            gTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGGTCATGGTTAACATTc                                                            <  1:201530/81‑1 (MQ=255)               cTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGGTCATGGTTAACATTCCgg                                                         <  1:201532/81‑1 (MQ=255)                     tgtTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGGTCATGGTTAACATTCCGGCCTAtt                                                   >  2:201562/1‑81 (MQ=255)                          gCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGGTCATGGTTAACATTCCGGCCTATTCGCt                                               <  1:201533/80‑1 (MQ=255)                                     ccAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGGTCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTAt                                     >  2:201556/1‑79 (MQ=255)                                      cAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGGTCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCa                                   <  1:201535/80‑1 (MQ=255)                                      cAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGGTCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAg                                  <  1:201534/81‑1 (MQ=255)                                       aGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGGTCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAg                                  >  2:201566/1‑80 (MQ=255)                                             tGCGCTTGCTGCCAACAGAGCTTTCTACCGGGGTCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGc                            >  1:201569/1‑80 (MQ=255)                                                         cAACAGAGCTTTCTACCGGGGTCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTgg               <  2:201536/81‑1 (MQ=255)                                                           aCAGAGCTTTCTACCGGGGTCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGAt             <  2:201537/81‑1 (MQ=255)                                                             agagCTTTCTACCGGGGTCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTc           >  1:201570/1‑81 (MQ=255)                                                                  tttCTACCGGGGTCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAg      >  1:201563/1‑81 (MQ=255)                                                                     cTACCGGGGTCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTc    <  1:201538/80‑1 (MQ=255)                                                                        ccGGGGTCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCAt  <  1:201539/79‑1 (MQ=255)                                                                             |                                                                          GCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCAT  >  NC_000913/982099‑982248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑