Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	153,179	C→T	V750I (GTT→ATT)	htrE ←	putative fimbrial usher protein HtrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	153,179	0	C	T	100.0%	126.7 / NA	35	V750I (GTT→ATT)	htrE	putative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/13);  total (22/13)

CTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCA  >  NC_000913/153101‑153252                                                                               |                                                                          cTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAatat                                                                         >  1:35465/1‑81 (MQ=255)  ttGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATc                                                                        >  1:35474/1‑81 (MQ=255)    gCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGt                                                                      >  2:35467/1‑81 (MQ=255)       cGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTtct                                                                   >  1:35483/1‑81 (MQ=255)        ggTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTtctc                                                                  <  2:35440/81‑1 (MQ=255)        ggTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTtct                                                                   >  2:35472/1‑80 (MQ=255)            tCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAg                                                               <  2:35441/80‑1 (MQ=255)              aaaaTCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTtctc                                                                  >  2:35477/1‑75 (MQ=255)              aaaaTCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTtctc                                                                  >  1:35489/1‑75 (MQ=255)                    aGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGat                                                      <  2:35442/81‑1 (MQ=255)                     gCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGata                                                     >  1:35471/1‑81 (MQ=255)                      cAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGatat                                                    >  2:35488/1‑81 (MQ=255)                       aaaGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATc                                                   >  2:35484/1‑81 (MQ=255)                        aaGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATcc                                                  <  2:35443/81‑1 (MQ=255)                          gacgacTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAg                                                >  1:35480/1‑81 (MQ=255)                          gacgacTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAg                                                >  2:35485/1‑81 (MQ=255)                             gacTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAgcgc                                             <  2:35444/81‑1 (MQ=255)                              acTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAgcgc                                             >  2:35494/1‑80 (MQ=255)                                  aaCCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATAc                                        <  1:35445/81‑1 (MQ=255)                                       tGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGttt                                   >  1:35487/1‑81 (MQ=255)                                         aCGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGttt                                   <  2:35446/79‑1 (MQ=255)                                            cgGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCAt                               >  2:35506/1‑80 (MQ=255)                                            cgGTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATg                              <  1:655342/81‑1 (MQ=255)                                             ggTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGa                             >  2:35522/1‑81 (MQ=255)                                             ggTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGa                             <  1:35447/81‑1 (MQ=255)                                              gTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGa                             >  1:35476/1‑80 (MQ=255)                                              gTACAGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGAt                            <  2:35448/81‑1 (MQ=255)                                                  aGCTACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAg                         >  2:35475/1‑80 (MQ=255)                                                     tACTGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGgag                      >  1:35464/1‑80 (MQ=255)                                                        tGCACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGgaga                     >  2:35482/1‑78 (MQ=255)                                                          cACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAaga                 <  2:35450/80‑1 (MQ=255)                                                          cACTGGTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTATCATGATAAGGAGAAaga                 <  2:35449/80‑1 (MQ=255)                                                               gTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCt            <  2:35451/80‑1 (MQ=255)                                                               gTACTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTg           >  2:35497/1‑81 (MQ=255)                                                                  cTTTTTAATTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGt         >  2:35503/1‑80 (MQ=255)                                                                         aTTCAATATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCa  >  1:35473/1‑80 (MQ=255)                                                                               |                                                                          CTTGCACGGTTTCAAAATCAGCAAAGACGACTGAACCCTGACGCGGTACAGCTACTGCACTGGTACTTTTTAATTCAACATCGTTCTCAAGATCGTTGATATCCAGCGCGATACGGTTTTCATGATAAGGAGAAAGAGCGCTGGTGACACCA  >  NC_000913/153101‑153252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑