Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	991,998	G→T	E459D (GAG→GAT)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	991,998	0	G	T	100.0%	95.3 / NA	27	E459D (GAG→GAT)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/16);  total (11/16)

TTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTT  >  NC_000913/991926‑992075                                                                         |                                                                              tttCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTacac                                                                       >  1:203185/1‑81 (MQ=255)  ttCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACAcc                                                                      >  1:203180/1‑81 (MQ=255)   tCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACAcc                                                                      <  2:203140/80‑1 (MQ=255)     cgccgTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCt                                                                    <  1:203141/80‑1 (MQ=255)      gccgTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGa                                                                  >  1:203174/1‑81 (MQ=255)        cgTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGAcc                                                                <  1:203142/81‑1 (MQ=255)          ttGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCAt                                                              <  2:203143/81‑1 (MQ=255)                cAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATcagcca                                                        <  1:203144/81‑1 (MQ=255)                cAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGGACACCCGGACCATcagcca                                                        <  1:203145/81‑1 (MQ=255)                 aGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATcagccag                                                       >  1:203176/1‑81 (MQ=255)                     aGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCa                                                   >  2:203177/1‑81 (MQ=255)                       tCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACg                                                 <  2:203146/81‑1 (MQ=255)                             acacCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACgccagc                                            >  2:203175/1‑80 (MQ=255)                              cacCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACgccagcc                                           <  1:203147/80‑1 (MQ=255)                                    ttGTGACCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCgg                                    <  2:203148/81‑1 (MQ=255)                                         aCCGTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAg                               <  2:203149/81‑1 (MQ=255)                                           cGTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAgg                              >  1:203187/1‑80 (MQ=255)                                            gTCAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCa                            <  1:203150/81‑1 (MQ=255)                                              cAAAGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAg                           >  1:203194/1‑80 (MQ=255)                                               aaaGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGaa                         <  1:203151/81‑1 (MQ=255)                                                aaGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATCAGCGAGCGCACGCCAGCCACGCCGGATCAGGCAGaa                         >  1:203203/1‑80 (MQ=255)                                                aaGACGACTACAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGaaa                        <  1:203152/81‑1 (MQ=255)                                                          cAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGc               <  2:203153/80‑1 (MQ=255)                                                          cAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCt              >  1:203181/1‑81 (MQ=255)                                                          cAATCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCt              >  2:203197/1‑81 (MQ=255)                                                           aaTCCGGAAACCGATCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTg             <  2:203154/81‑1 (MQ=255)                                                                      cGATCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGtt  <  2:203155/81‑1 (MQ=255)                                                                         |                                                                              TTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTT  >  NC_000913/991926‑992075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑