Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,037,262	C→T	H172Y (CAT→TAT)	hyaF →	protein HyaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,037,262	0	C	T	100.0%	107.7 / NA	30	H172Y (CAT→TAT)	hyaF	protein HyaF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/22);  total (8/22)

CAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAG  >  NC_000913/1037185‑1037339                                                                              |                                                                              cAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATg                                                                            <  2:211021/81‑1 (MQ=255)  aaCGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTAt                                                                             >  2:211074/1‑79 (MQ=255)    cGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGt                                                                         <  1:211022/81‑1 (MQ=255)        aaaTCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACt                                                                     <  1:211023/81‑1 (MQ=255)        aaaTCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACt                                                                     <  2:211024/81‑1 (MQ=255)           tctcTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTgg                                                                   >  2:211067/1‑80 (MQ=255)               ttGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGcac                                                                <  1:211026/79‑1 (MQ=255)               ttGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGc                                                                  <  2:211025/77‑1 (MQ=255)                  ccGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGt                                                            <  1:211028/80‑1 (MQ=255)                  ccGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTa                                                           <  2:211027/81‑1 (MQ=255)                     aCAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGt                                                        <  1:211030/81‑1 (MQ=255)                     aCAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGt                                                        <  2:211029/81‑1 (MQ=255)                       aGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGTaa                                                      >  1:211070/1‑81 (MQ=255)                        gATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGTAAc                                                     <  1:211031/81‑1 (MQ=255)                              cTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGAc                                               <  2:211032/81‑1 (MQ=255)                                          cccATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGcac                                   <  2:211033/81‑1 (MQ=255)                                            cATTGATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAg                                 >  1:211075/1‑81 (MQ=255)                                                gATGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCAt                              >  2:211087/1‑80 (MQ=255)                                                 aTGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCAtt                             <  1:211034/80‑1 (MQ=255)                                                 aTGGCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCAtt                             <  2:211035/80‑1 (MQ=255)                                                    gCCTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATc                         >  2:211072/1‑81 (MQ=255)                                                      cTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTg                       <  1:211038/81‑1 (MQ=255)                                                      cTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTg                       <  2:211036/81‑1 (MQ=255)                                                      cTGATGAATGGCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTg                       <  2:211037/81‑1 (MQ=255)                                                                gCCTACCGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTa              <  1:211039/80‑1 (MQ=255)                                                                    aCCGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCAt         >  2:211077/1‑81 (MQ=255)                                                                      cGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCAt         >  2:211082/1‑79 (MQ=255)                                                                      cGTTGGCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCa       <  2:211040/81‑1 (MQ=255)                                                                          ggCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCg    <  2:211042/80‑1 (MQ=255)                                                                          ggCGTATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCg    <  1:211041/80‑1 (MQ=255)                                                                           gcgtATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAg  <  1:211043/81‑1 (MQ=255)                                                                              |                                                                              CAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAG  >  NC_000913/1037185‑1037339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑