Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,047,912	C→A	A12S (GCC→TCC)	gfcD ←	putative lipoprotein GfcD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,047,912	0	C	A	100.0%	108.4 / NA	30	A12S (GCC→TCC)	gfcD	putative lipoprotein GfcD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/15);  total (15/15)

AAAGTCTGATTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGCCAGGCAGCTTAAAAGATAAGAATTCTTCTTCATCAGTCAGGAACACGTTGCGTTAAAACAGACACAATCTGGTCATT  >  NC_000913/1047835‑1047989                                                                              |                                                                              aaaGTCTGATTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAg                                                                            >  2:212987/1‑81 (MQ=255)  aaGTCTGATTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAgg                                                                           >  2:212979/1‑81 (MQ=255)    gTCTGATTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCa                                                                         <  1:212946/81‑1 (MQ=255)       tGATTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCt                                                                      >  2:212977/1‑81 (MQ=255)        gATTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCt                                                                      >  1:212983/1‑80 (MQ=255)         aTTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCtt                                                                     <  2:212949/80‑1 (MQ=255)         aTTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCt                                                                      <  1:212948/79‑1 (MQ=255)         aTTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCt                                                                      <  1:212947/79‑1 (MQ=255)            gCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCTTaa                                                                   >  2:212973/1‑79 (MQ=255)                    cccAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGaa                                                         >  1:212968/1‑81 (MQ=255)                    cccAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGaa                                                         >  2:212988/1‑81 (MQ=255)                      cAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAAtt                                                       >  2:212992/1‑81 (MQ=255)                       aGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAAttc                                                      <  2:212950/81‑1 (MQ=255)                       aGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAAttc                                                      <  2:212952/81‑1 (MQ=255)                       aGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAAttc                                                      <  1:212951/81‑1 (MQ=255)                          ggATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAAttcttc                                                   <  2:212953/81‑1 (MQ=255)                            aTCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAAttcttctt                                                 >  2:212993/1‑81 (MQ=255)                                gggTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAATTCTTCTtcat                                              >  2:212981/1‑80 (MQ=255)                                     ggTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAATTCTTCTTCAtcagtc                                         <  1:212954/80‑1 (MQ=255)                                          aTACTTCAGCATGACAGGCACTGGAGACGGCAATTGACAGGCAGCTTAAAAGATAAGAATTCTTCTTCATCAGTCAGGAac                                   <  2:212955/81‑1 (MQ=255)                                                    aTGACAGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAATTCTTCTTCATCAGTCAGGAACACGTTGCGtt                         <  2:212956/81‑1 (MQ=255)                                                       aCAGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAATTCTTCTTCATCAGTCAGGAACACGTTGCGTTaa                       <  1:212957/80‑1 (MQ=255)                                                         aGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAATTCTTCTTCATCAGTCAGGAACACGTTGCGTTAAAAc                    <  2:212958/81‑1 (MQ=255)                                                         aGGCACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAATTCTTCTTCATCAGTCAGGAACACGTTGCGTTAAAAc                    <  1:212959/81‑1 (MQ=255)                                                            cACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAATTCTTCTTCATCAGTCAGGAACACGTTGCGTTAAAACAGa                 >  1:212989/1‑81 (MQ=255)                                                            cACTGGAGACGGCAATGGACAGGCAGCTTAAAAGATAAGAATTCTTCTTCATCAGTCAGGAACACGTTGCGTTAAAACAGa                 >  2:213001/1‑81 (MQ=255)                                                                 gagaCGGCAATGGACAGGCAGCTTAAAAGATAAGAATTCTTCTTCATCAGTCAGGAACACGTTGCGTTAAAACAGacac              <  2:212960/79‑1 (MQ=255)                                                                    aCGGCAATGGACAGGCAGCTTAAAAGATAAGAATTCTTCTTCATCAGTCAGGAACACGTTGCGTTAAAACAGACACAATCt         >  2:213017/1‑81 (MQ=255)                                                                          aTGGACAGGCAGCTTAAAAGATAAGAATTCTTCTTCATCAGTCAGGAACACGTTGCGTTAAAACAGACACAATCTGGTCAt   >  2:212997/1‑81 (MQ=255)                                                                           tGGACAGGCAGCTTAAAAGATAAGAATTCTTCTTCATCAGTCAGGAACACGTTGCGTTAAAACAGACACAATCTGGTCAtt  >  2:212985/1‑81 (MQ=255)                                                                              |                                                                              AAAGTCTGATTGCGACGAACCCAGCGGATCCGGGTAGGTTAATACTTCAGCATGACAGGCACTGGAGACGGCAATGGCCAGGCAGCTTAAAAGATAAGAATTCTTCTTCATCAGTCAGGAACACGTTGCGTTAAAACAGACACAATCTGGTCATT  >  NC_000913/1047835‑1047989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑