Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,064,853	A→T	D273V (GAT→GTT)	yccE →	uncharacterized protein YccE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,064,853	0	A	T	100.0%	38.8 / NA	12	D273V (GAT→GTT)	yccE	uncharacterized protein YccE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (5/7);  total (5/7)

TAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTAAAAAA  >  NC_000913/1064776‑1064923                                                                              |                                                                       tAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCt                                                                     <  2:215893/81‑1 (MQ=255)         cGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCt                                                              >  1:326956/1‑80 (MQ=255)                ggCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAAcc                                                       >  2:215913/1‑80 (MQ=255)                ggCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCa                                                      <  2:215894/81‑1 (MQ=255)                                  aTTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGagtag                                    >  2:215911/1‑81 (MQ=255)                                               aGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCAt                         <  1:215895/79‑1 (MQ=255)                                               aGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATgg                       <  2:215897/81‑1 (MQ=255)                                               aGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATg                        <  2:215896/80‑1 (MQ=255)                                                        gTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTg              >  2:215912/1‑81 (MQ=255)                                                               cGTTATTTGGCGTTGTTCTACAATCTGACAACAAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTa       <  1:215898/81‑1 (MQ=255)                                                                  tATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTaaa     >  1:215908/1‑80 (MQ=255)                                                                    tttGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTaaaaaa  <  2:215899/81‑1 (MQ=255)                                                                              |                                                                       TAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTAAAAAA  >  NC_000913/1064776‑1064923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑