BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,065,842	G→T	M86I (ATG→ATT)	agp →	glucose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,065,842	0	G	T	100.0%	98.1 / NA	27	M86I (ATG→ATT)	agp	glucose‑1‑phosphatase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (8/19); total (8/19)

TGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCA  >  NC_000913/1065765‑1065918

tGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGt                                                                           <  1:215985/81‑1 (MQ=255)

   ccAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGaa                                                                        <  2:215986/81‑1 (MQ=255)

    cAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAAt                                                                       <  2:215987/81‑1 (MQ=255)

       aaTGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAAtggc                                                                    <  1:215988/81‑1 (MQ=255)

       aaTGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAAtggc                                                                    >  2:216030/1‑81 (MQ=255)

                tCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGc                                                           <  1:215989/81‑1 (MQ=255)

                    cGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAggg                                                       <  2:215990/81‑1 (MQ=255)

                       taggCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGAt                                                    <  1:215991/79‑1 (MQ=255)

                          gCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGt                                                 >  1:216033/1‑81 (MQ=255)

                           cAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTg                                                <  2:215993/81‑1 (MQ=255)

                           cAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTg                                                <  2:215992/81‑1 (MQ=255)

                             aCTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGaa                                              <  1:215994/81‑1 (MQ=255)

                                   caccaAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCggg                                        <  2:215995/81‑1 (MQ=255)

                                    accaAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCgggg                                       >  1:216032/1‑81 (MQ=255)

                                      caAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGa                                     <  2:215996/81‑1 (MQ=255)

                                           gTGGCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGcc                                <  2:215997/81‑1 (MQ=255)

                                             ggCGTGCTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCccg                              >  1:216034/1‑81 (MQ=255)

                                                  gCTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCccgccgcc                         >  1:216041/1‑81 (MQ=255)

                                                   cTCGAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCccgccgcc                         <  1:215998/80‑1 (MQ=255)

                                                      gAAGTGTATATGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTa                      >  1:216040/1‑80 (MQ=255)

                                                            tataTGGGCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGtt               <  1:215999/81‑1 (MQ=255)

                                                                   gCCATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTtatgcct        <  1:216000/81‑1 (MQ=255)

                                                                    ccATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTtatgccta       <  2:216001/81‑1 (MQ=255)

                                                                     cATTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTtatgcctat      >  1:216048/1‑81 (MQ=255)

                                                                      aTTACATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTtatgcctatg     >  1:216047/1‑81 (MQ=255)

                                                                         aCATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTtatgcctatgcc   <  2:216002/80‑1 (MQ=255)

                                                                          cATTCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCa  <  1:216003/80‑1 (MQ=255)

TGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCA  >  NC_000913/1065765‑1065918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`