Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,069,550	G→A	A97V (GCG→GTG)	rutG ←	pyrimidine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,069,550	0	G	A	100.0%	70.5 / NA	20	A97V (GCG→GTG)	rutG	pyrimidine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/8);  total (12/8)

AGCCCACAGGCGATAATCCCGCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAAC  >  NC_000913/1069478‑1069627                                                                         |                                                                              aGCCCACAGGCGATAATCCCGCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATc                                                                       <  2:216605/81‑1 (MQ=255)  gCCCACAGGCGATAATCCCGCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATca                                                                      >  1:216632/1‑81 (MQ=255)   cccACAGGCGATAATCCCGCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATcac                                                                     >  1:216636/1‑81 (MQ=255)    ccACAGGCGATAATCCCGCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATcacc                                                                    <  1:216606/81‑1 (MQ=255)      acaGGCGATAATCCCGCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATcaccac                                                                  >  1:216646/1‑81 (MQ=255)        aGGCGATAATCCCGCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATCACCACGc                                                                <  1:216607/81‑1 (MQ=255)           cGATAATCCCGCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATCACCACGCCGa                                                             >  2:216628/1‑81 (MQ=255)                 tCCCGCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATCACCACGCCGACAAAAg                                                       >  2:216629/1‑81 (MQ=255)                        cAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATCACCACGCCGACAAAAGCCGCGCt                                                <  1:216608/81‑1 (MQ=255)                            gcAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATCACCACGCCGACAAAAGCCGCGCTGGAg                                            >  1:216635/1‑81 (MQ=255)                             cAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGc                                           <  2:216609/81‑1 (MQ=255)                              aaTGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGcc                                          >  2:216634/1‑81 (MQ=255)                                tGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGcc                                          >  1:216631/1‑79 (MQ=255)                                          cGGGTTAATGCCCTGACCGTTAAAGCCCGTCACGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTggg                              >  1:216643/1‑81 (MQ=255)                                                    cccTGACCGTTAAAGCCCGTCACGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGcgccc                     <  1:216610/80‑1 (MQ=255)                                                          ccGTTAAAGCCCGTCACGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGta              <  1:216611/81‑1 (MQ=255)                                                            gTTAAAGCCCGTCACGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGtaa             >  1:216637/1‑80 (MQ=255)                                                                 aGCCCGTCACGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAg        >  1:216642/1‑80 (MQ=255)                                                                      gTCACGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGaaaaa   >  1:216664/1‑80 (MQ=255)                                                                      gTCACGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAAc  <  2:216612/81‑1 (MQ=255)                                                                         |                                                                              AGCCCACAGGCGATAATCCCGCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAAC  >  NC_000913/1069478‑1069627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑