Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,080,669	T→C	I455I (ATT→ATC)	putP →	proline:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,080,669	0	T	C	100.0%	105.9 / NA	30	I455I (ATT→ATC)	putP	proline:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/19);  total (11/19)

GCTGGCGGGGATGATCATCGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATTATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATG  >  NC_000913/1080594‑1080744                                                                            |                                                                            gcTGGCGGGGATGATCATCGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTcc                                                                        <  1:218626/81‑1 (MQ=255)  cTGGCGGGGATGATCATCGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCg                                                                       >  1:218667/1‑81 (MQ=255)  cTGGCGGGGATGATCATCGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCg                                                                       <  2:218627/81‑1 (MQ=255)       ggggATGATCATCGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCtt                                                                  <  2:218628/81‑1 (MQ=255)          gatgatCATCGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTAt                                                               <  2:218629/81‑1 (MQ=255)           atgatCATCGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATc                                                              >  2:218671/1‑81 (MQ=255)           atgatCATCGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATc                                                              <  2:218630/81‑1 (MQ=255)              atcatcGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCtt                                                            <  1:218631/80‑1 (MQ=255)              atcatcGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCtt                                                            <  2:218635/80‑1 (MQ=255)              atcatcGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCtt                                                            <  2:218634/80‑1 (MQ=255)              atcatcGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCtt                                                            <  2:218632/80‑1 (MQ=255)              atcatcGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCTTc                                                           <  1:218633/81‑1 (MQ=255)                 atcGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCTTCGGc                                                        <  2:218636/81‑1 (MQ=255)                       gcgcTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCTTCGGCAGTAtt                                                  >  2:218686/1‑81 (MQ=255)                        cgcTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCTTCGGCAGTATTg                                                 <  1:218637/81‑1 (MQ=255)                                gTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTa                                         <  1:218638/81‑1 (MQ=255)                                  tATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAgt                                       >  2:218677/1‑81 (MQ=255)                                   aTCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAgtg                                      >  2:218669/1‑81 (MQ=255)                                    tCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAgtgt                                     <  2:218639/81‑1 (MQ=255)                                      gTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGttt                                   <  2:218640/81‑1 (MQ=255)                                               cAGTTCGGCTGGCTGGGACTGTACGAATTCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTg                          <  2:218641/81‑1 (MQ=255)                                                   tCGGCTGGCTGGGACTGTACGAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGt                       >  1:218674/1‑80 (MQ=255)                                                       ctggctggGACTGTACGAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAgc                  >  1:218676/1‑81 (MQ=255)                                                         ggctggGACTGTACGAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAgcgc                >  1:218681/1‑81 (MQ=255)                                                             gggACTGTACGAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGt             >  2:218682/1‑80 (MQ=255)                                                              ggACTGTACGAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGt             <  1:218642/79‑1 (MQ=255)                                                                aCTGTACGAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAgc         >  1:218680/1‑81 (MQ=255)                                                                      cGAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGAt   <  2:218643/81‑1 (MQ=255)                                                                       gAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATg  <  1:218644/81‑1 (MQ=255)                                                                       gAAATCATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATg  >  2:218673/1‑81 (MQ=255)                                                                            |                                                                            GCTGGCGGGGATGATCATCGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATTATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATG  >  NC_000913/1080594‑1080744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑