Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,096,234	C→G	P131R (CCG→CGG)	ycdU →	uncharacterized protein YcdU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,096,234	0	C	G	100.0%	59.8 / NA	18	P131R (CCG→CGG)	ycdU	uncharacterized protein YcdU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (10/8);  total (10/8)

CAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCCGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCG  >  NC_000913/1096165‑1096304                                                                      |                                                                       cAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATc                                                              <  2:220929/80‑1 (MQ=255)      aGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTct                                                         <  2:220930/80‑1 (MQ=255)        ttGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTctgct                                                      >  2:220953/1‑81 (MQ=255)                 ataCCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGgat                                             >  1:220956/1‑81 (MQ=255)                      aGCTTCTACCCTCGTTATATGCGCTGTTCCTAATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATcc                                        <  2:220931/81‑1 (MQ=255)                           cTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCAc                                    >  2:220951/1‑80 (MQ=255)                                   gTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGt                           >  2:220952/1‑81 (MQ=255)                                   gTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGt                           >  2:220945/1‑81 (MQ=255)                                    ttATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTc                          <  1:220932/81‑1 (MQ=255)                                         tGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAg                       >  2:220961/1‑79 (MQ=255)                                          gcgcTGTTCCTGATTTCGTTACTTTGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTAAAGCAg                    >  2:220988/1‑81 (MQ=255)                                                ttCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCt              <  1:220934/81‑1 (MQ=255)                                                ttCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCt              <  2:220933/81‑1 (MQ=255)                                                  ccTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTAt            >  1:220971/1‑81 (MQ=255)                                                   cTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTAt            <  2:220935/80‑1 (MQ=255)                                                         tCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAg       >  2:220966/1‑79 (MQ=255)                                                           gTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGc   <  2:220936/81‑1 (MQ=255)                                                             tACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCg  >  2:220962/1‑80 (MQ=255)                                                                      |                                                                       CAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCCGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCG  >  NC_000913/1096165‑1096304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑